Evidence map›Paper›PMID 41648072›Full record

ArticleRegenerative therapy2026

Intra-articular injections of platelet-rich plasma suppress cartilage degeneration in a mouse model of knee osteoarthritis.

Yasumasa Momoi, Yoshitomo Saita, Ryosuke Nakajima, Sayuri Uchino, Hirofumi Nishio, Shin Fukusato, Takanori Wakayama, Yohei Kobayashi, Atsushi Furuhata, Hiroshi Ikeda and 2 more

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Article in Regenerative therapy, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

12 authors.

Yasumasa MomoiDepartment of Orthopaedics, Juntendo University, Faculty of Medicine, 2-1-1 Hongo, Bunkyo-ku, Tokyo, Japan.
Yoshitomo SaitaDepartment of Orthopaedics, Juntendo University, Faculty of Medicine, 2-1-1 Hongo, Bunkyo-ku, Tokyo, Japan.
Ryosuke NakajimaDepartment of Orthopaedics, Juntendo University, Faculty of Medicine, 2-1-1 Hongo, Bunkyo-ku, Tokyo, Japan.
Sayuri UchinoDepartment of Orthopaedics, Juntendo University, Faculty of Medicine, 2-1-1 Hongo, Bunkyo-ku, Tokyo, Japan.
Hirofumi NishioDepartment of Orthopaedics, Juntendo University, Faculty of Medicine, 2-1-1 Hongo, Bunkyo-ku, Tokyo, Japan.
Shin FukusatoDepartment of Orthopaedics, Juntendo University, Faculty of Medicine, 2-1-1 Hongo, Bunkyo-ku, Tokyo, Japan.
Takanori WakayamaDepartment of Orthopaedics, Juntendo University, Faculty of Medicine, 2-1-1 Hongo, Bunkyo-ku, Tokyo, Japan.
Yohei KobayashiDepartment of Orthopaedics, Juntendo University, Faculty of Medicine, 2-1-1 Hongo, Bunkyo-ku, Tokyo, Japan.
Atsushi FuruhataLaboratory of Morphology and Image Analysis, Juntendo University Graduate School of Medicine, 2-1-1 Hongo, Bunkyo-ku, Tokyo, Japan.
Hiroshi IkedaDepartment of Orthopaedics, Juntendo University, Faculty of Medicine, 2-1-1 Hongo, Bunkyo-ku, Tokyo, Japan.
Kazuo KanekoDepartment of Orthopaedics, Juntendo University, Faculty of Medicine, 2-1-1 Hongo, Bunkyo-ku, Tokyo, Japan.
Muneaki IshijimaDepartment of Orthopaedics, Juntendo University, Faculty of Medicine, 2-1-1 Hongo, Bunkyo-ku, Tokyo, Japan.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Introduction: Pain-relieving drugs, such as nonsteroidal anti-inflammatory drugs and hyaluronic acid, are standard treatments for knee osteoarthritis (OA); however, no disease-modifying drugs exist for knee OA. Platelet-rich plasma (PRP) is a novel treatment with both symptom improvement and disease-modifying effects; however, the underlying mechanism remains unknown. In addition, the biologically active substances contained in PRP differ greatly depending on the purification method used. This controlled laboratory study aimed to investigate the therapeutic effects of two types of PRP with different white blood cell concentrations on knee OA using an animal model. Methods: Leukocyte-rich PRP (LR-PRP) and leukocyte-poor PRP (LP-PRP) were prepared from 10-week-old female C57BL/6 mice. A mouse model of knee osteoarthritis was generated by the unilateral transection of the medial meniscus in the right hind limb. Mice were randomly assigned to three treatment groups that received 6 μL intra-articular injections of either phosphate-buffered saline (control), LR-PRP, or LP-PRP at 2-, 4-, and 6-weeks post-surgery. Mice were sacrificed 12 weeks post-surgery and histologic analysis, immunohistochemistry analysis for CD68 and three-dimensional micro-computed tomography (3DμCT) of knee joints were analyzed. Hind limb weight-bearing distribution was measured preoperatively and at 4- and 12-weeks post-surgery. Statistical analyses were performed using GraphPad Prism 9.0.2. P-values of <5 % were considered statistically significant. Results: Histological analysis of the femoral medial condyle (Osteoarthritis Research Society International (OARSI) score) showed that both the LP and LR groups had significantly suppressed cartilage destruction compared with the Phosphate Buffered Saline (PBS) group (P = 0.01). The percentage of CD68-positive synovial macrophages in the lateral joint was significantly lower in the LR group than in the PBS group (PBS: 2.0 ± 2.9 %, LR: 0.6 ± 1.3 %, LP: 0.9 ± 1.9 %; P = 0.02). The affected-side load rate (%) increased in the LR and LP groups, with a significant increase observed in the LR group from week four. PBS group, Pre/4w/12w = 36.0 ± 4.8/33.8 ± 6.8/36.2 ± 7.4 % (P = 0.60); LR group, Pre/4w/12w = 31.9 ± 2.2/34.3 ± 8.3/44.2 ± 4.8 % (P < 0.01); LP group, Pre/4w/12w = 33.5 ± 7.4/36.1 ± 7.4/42.0 ± 5.2 % (P = 0.02). Conversely, no significant difference in BMD was observed between groups. Conclusions: Intra-articular injection of LR- and LP-PRP attenuated cartilage degeneration in the medial femoral condyle in a mouse model of knee osteoarthritis.

Indexed as

Articular cartilageCell/molecular biologyGrowth factors/Healing enhancementKneePlatelet rich plasma

Identifiers

PMID41648072
PMCPMC12870470

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.