Evidence map›Paper›PMID 41646724›Full record

ArticlemedRxiv : the preprint server for health sciences2026

Multimodal single-cell and spatial profiling reveals altered T cell-mediated immunity and B-cell follicular architecture in non-metastatic lymph nodes of patients with aggressive non-small cell lung cancer.

Zhan Hao Xi, Yusuke Koga, Shannon McDermott, Erin E Kane, Roxanna Pfefferkorn, Ehab Billatos, Paul R Hosking, Jennifer E Beane, Eric J Burks, Sarah A Mazzilli and 2 more

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in medRxiv : the preprint server for health sciences, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Zhan Hao XiDepartment of Medicine, Section of Computational Biomedicine, Boston University Chobanian & Avedisian School of Medicine, Boston, MA, USA.
Yusuke KogaDepartment of Medicine, Section of Computational Biomedicine, Boston University Chobanian & Avedisian School of Medicine, Boston, MA, USA.
Shannon McDermottDepartment of Medicine, Section of Computational Biomedicine, Boston University Chobanian & Avedisian School of Medicine, Boston, MA, USA.
Erin E KaneDepartment of Medicine, Section of Computational Biomedicine, Boston University Chobanian & Avedisian School of Medicine, Boston, MA, USA.
Roxanna PfefferkornDepartment of Medicine, Section of Computational Biomedicine, Boston University Chobanian & Avedisian School of Medicine, Boston, MA, USA.
Ehab BillatosDepartment of Medicine, Section of Computational Biomedicine, Boston University Chobanian & Avedisian School of Medicine, Boston, MA, USA.
Paul R HoskingDepartment of Pathology& Laboratory Medicine, University of Rochester Medical Center, Rochester, NY, USA.
Jennifer E BeaneDepartment of Medicine, Section of Computational Biomedicine, Boston University Chobanian & Avedisian School of Medicine, Boston, MA, USA.
Eric J BurksDepartment of Pathology & Laboratory Medicine, Boston University Chobanian & Avedisian School of Medicine, Boston Medical Center, Boston, MA, USA.ORCID 0000-0002-3122-2875
Sarah A MazzilliDepartment of Medicine, Section of Computational Biomedicine, Boston University Chobanian & Avedisian School of Medicine, Boston, MA, USA.
Kei SuzukiINOVA, Department of Surgery, Division of Thoracic Surgery, Falls Church, VA, USA.
Joshua D CampbellDepartment of Medicine, Section of Computational Biomedicine, Boston University Chobanian & Avedisian School of Medicine, Boston, MA, USA.

Funding

The Lung PCA: A Multi-Dimensional Atlas of Pulmonary PremalignancyU2CCA233238 · NCI · BOSTON UNIVERSITY MEDICAL CAMPUS · PI SPIRA, AVRUM E · 2018 to 2021
$7.0M
An interactive analysis toolkit for single cell RNA-seq in cancer researchU01CA220413 · NCI · BOSTON UNIVERSITY MEDICAL CAMPUS · PI JOHNSON, WILLIAM EVAN · 2017 to 2019
$1.2M
Integrative clustering of cells and samples using multi-modal single-cell dataR01LM013154 · NLM · BOSTON UNIVERSITY MEDICAL CAMPUS · PI CAMPBELL, JOSHUA D, YAJIMA, MASANAO · 2019 to 2021
$1.1M
NCI NIH HHS U01 CA220413NCI NIH HHS U2C CA233238NLM NIH HHS R01 LM013154
6 · The paper itself

Abstract

Regional lymph nodes (LNs) in the thoracic cavity serve as essential immunological hubs that coordinate humoral and cell-mediated responses against the development and progression of non-small cell lung cancer (NSCLC). To investigate immune dysregulation in the non-metastatic regional LNs of patients with aggressive NSCLC, we performed multimodal profiling on 36 LNs from 11 patients undergoing curative-intent resection including CITE-seq, scRNA-seq, and Imaging Mass Cytometry (IMC). Regional N1 LNs from patients with more aggressive disease (stage IB-IIIA) exhibited a significant enrichment of dysfunctional CD8

Identifiers

PMID41646724
PMCPMC12870645

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.