Evidence map›Paper›PMID 41646385›Full record

ArticleResearch square2026

Soaking Up Success: Sponge-Assisted Nanoparticle Transfection.

Yevgeny Brudno, Rukesh Chinthapatla, Hayden Sandt, Israt Jahan Tulip, Vedamudra Kolluru, Hannah Haynes, Savannah Muron, Sharda Pandit, Christopher Moody, Madelyn VanBlunk and 4 more

Abstract readPreprint
In one paragraph

Article in Research square, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Yevgeny BrudnoSchool of Pharmacy at University of North Carolina at Chapel Hill.ORCID https://orcid.org/0000-0003-2963-3293
Rukesh ChinthapatlaUniversity of North Carolina at Chapel Hill.
Hayden SandtUniversity of North Carolina at Chapel Hill.
Israt Jahan TulipUniversity of North Carolina at Chapel Hill.
Vedamudra KolluruUniversity of North Carolina at Chapel Hill.
Hannah HaynesUniversity of North Carolina at Chapel Hill.
Savannah MuronUniversity of North Carolina at Chapel Hill.
Sharda PanditUniversity of North Carolina at Chapel Hill.
Christopher MoodyUniversity of North Carolina at Chapel Hill.
Madelyn VanBlunkUniversity of North Carolina at Chapel Hill.
Soumen SahaDuke University.
Ashutosh ChilkotiDuke University.ORCID https://orcid.org/0000-0002-1569-2228
Erin HeinzenUniversity of North Carolina at Chapel Hill.
Pritha AgarwallaUniversity of North Carolina at Chapel Hill.

Funding

Virology Research Program (Program 4)P30CA016086 · NCI · UNIV OF NORTH CAROLINA CHAPEL HILL · PI Deborah F. Tate · 1985 to 2026
$201.5M
NRSA in GeneticsT32GM135128 · NIGMS · UNIV OF NORTH CAROLINA CHAPEL HILL · PI Daniel J McKay, JEFF J. SEKELSKY · 2020 to 2026
$5.1M
Substrate Mediated siRNA Delivery from Scaffolds to Promote Wound RepairR01EB019409 · NIBIB · VANDERBILT UNIVERSITY · PI Craig Lewis Duvall · 2014 to 2026
$4.0M
Biomaterial Scaffolds for Ex Vivo and In Situ CAR-T Cell ProductionR37CA260223 · NCI · UNIV OF NORTH CAROLINA CHAPEL HILL · PI Yevgeny Brudno · 2021 to 2026
$2.5M
MASTER Scaffolds for Rapid, Single-Step Manufacture and Prototyping of CAR-T cellsR33CA281875 · NCI · UNIV OF NORTH CAROLINA CHAPEL HILL · PI Yevgeny Brudno · 2023 to 2026
$1.3M
NCI NIH HHS P30 CA016086NCI NIH HHS R33 CA281875NCI NIH HHS R37 CA260223NIBIB NIH HHS R01 EB019409NIGMS NIH HHS T32 GM135128
6 · The paper itself

Abstract

Current cell transfection systems face efficiency limitations, particularly for challenging cell types. We present a sponge-assisted transfection method enabling efficient mRNA transfection at nanogram-scale doses-a several orders of magnitude-reduction compared to the microgram quantities typically required by lipid-based and membrane-disruption methods. This approach achieves robust, dose-dependent transfection across difficult-to-transfect cell types, establishing broad applicability for both basic research and therapeutic use.

Identifiers

PMID41646385
PMCPMC12869581

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.