Evidence map›Paper›PMID 41645841›Full record

ArticleBiotechnology journal2026

Mycoponics: Controlled Bioproduction Utilizing Biophysical, Solid-State, Liquid Nutrient Delivery.

D Marshall Porterfield, Simone X Moulton, Adriana K Sanchez, Anna Sorg, Tayla Koenig, M Shane Terrell, Sigrid Zahner, Alex Baena, Caitlin Proctor, Richard J Barker

Abstract read
In one paragraph

Article in Biotechnology journal, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

D Marshall PorterfieldDepartment of Agricultural and Biological Engineering, Purdue University, West Lafayette, Indiana, USA.
Simone X MoultonSchool of Aeronautics & Astronautics, Purdue University, West Lafayette, Indiana, USA.
Adriana K SanchezDepartment of Agricultural and Biological Engineering, Purdue University, West Lafayette, Indiana, USA.
Anna SorgNatural Resources and Environmental Science Program, Purdue University, West Lafayette, Indiana, USA.
Tayla KoenigDepartment of Agricultural and Biological Engineering, Purdue University, West Lafayette, Indiana, USA.
M Shane TerrellDepartment of Art and Design, Purdue University, West Lafayette, Indiana, USA.
Sigrid ZahnerDepartment of Art and Design, Purdue University, West Lafayette, Indiana, USA.
Alex BaenaDepartment of Agricultural and Biological Engineering, Purdue University, West Lafayette, Indiana, USA.
Caitlin ProctorDepartment of Agricultural and Biological Engineering, Purdue University, West Lafayette, Indiana, USA.
Richard J BarkerDepartment of Agricultural and Biological Engineering, Purdue University, West Lafayette, Indiana, USA.

Funding

DMP
6 · The paper itself

Abstract

Mycoponic biotechnology, inspired by hydroponics-a vital technology for agriculture research and space exploration, is limited by innate substrate contamination commonplace in commercial mycoproduction. Using micro-structured ceramic tubes as "substrate," mycoponics provides mycelial cellular filaments with an air-phase/solid-state, antimicrobial interface for cellular liquid nutrient media uptake. We show how antimicrobial biophysical size exclusion facilitates mycoponic "persistent-filtration-defense" (PFD), experimentally confirmed using flow cytometry, and electron microscopic analysis of the interfacial ceramic pores (less than 300 nm). The antimicrobial mycoponic interface enabled development of a complete mycoponic nutrient medium, producing blue oyster mushrooms from liquid culture 2 weeks post inoculation. This completely eliminates grain-spawn (2-4 weeks) and fruiting phase (2-4 weeks) times using granular substrate bags that require energy/time-intensive antimicrobial processing. Mycelial colonization times decreased by 9 days, while biomass increased (170%) with activated carbon inside the tubes. Mycoponic cultivation of Reishi mycoleather gloves demonstrates direct 3D-mycomaterials, and we show how mycoponics enables advanced scientific imaging (thermal) and techniques, including mycelial exudate recovery for drug discovery. Mycoponics enables hybrid solid-state, liquid culture for continuous bioproduction of mycelial pharmaceuticals, representing 18% of the global market. The efficiency and extended cultivation enabled by mycoponics will facilitate significant future advances in mycology and mycoengineering.

Indexed as

BiotechnologyCulture MediaBioreactorsMyceliumCulture Mediabioproductionmyceliamycologymycoponics

Identifiers

PMID41645841
PMCPMC12878557

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.