ArticleAdvanced science (Weinheim, Baden-Wurttemberg, Germany)2026
Microfluidic-Driven Lipid Nanoparticles for Improved miRNA Delivery via Endo-Lysosomal Trafficking Optimization.
Article in Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
2 citing papers in PubMed.
- Acoustically Activatable Drug-Loaded Nanodroplets for Mechanochemical Therapy in Solid Tumors.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Effects of different mixing techniques on mRNA lipid nanoparticle physicochemistry and biological performance.Nature communications · 2026Article
Corrections and comments
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Authors and funding
9 authors.
Funding
Abstract
This study investigates the influence of post-processing techniques on lipid nanoparticles (LNPs) designed for miRNA delivery in in vitro transfection models. We compared blank and miRNA-loaded LNPs (LNP-miRNA) in terms of size, polydispersity index, zeta potential, electrophoretic mobility, and conductivity. miRNA encapsulation increases lipid particle size by 43.6%, due to structural rearrangements. Post-processing methods, including sonication, filtration, dialysis, and thermal treatment, significantly alter particle characteristics. Sonication and filtration decrease particle size and improve uniformity, enhancing colloidal stability. Dialysis further refines the particle size but decreases its electrophoretic mobility. Non-dialyzed, sonicated, and filtered LNP-miRNA samples demonstrate the most favorable electrokinetic profile, maintaining low conductivity (0.003 mS/cm) and high electrophoretic mobility (3.16 ± 0.22 µm cm/V·s), suggesting optimal stability for gene delivery. Zeta potential measurements show that sonication and filtration increase the surface charge of LNP-miRNA formulations from +18.9 to +29.3 mV, enhancing colloidal stability, while dialysis reduces it to +1.9 mV. Although sonicated and filtered LNP-miRNA samples exhibited more favorable physicochemical properties, the dialyzed formulations modulate intracellular trafficking, resulting in earlier intracellular availability and prolonged persistence of delivered miRNA. This work establishes a framework for optimizing non-viral miRNA delivery by showing how post-processing shapes LNP stability and transfection performance.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.