Evidence map›Paper›PMID 41645262›Full record

ArticleMicrobiome2026

Altered rumen bacterial flora is associated with increased lipogenesis of adipose tissue in obese dairy cows before calving.

Chenxu Li, Guowen Liu, Yuting Yang, Zhaoxin Shi, Qi Shao, Zhiyuan Fang, Yuxiang Song, Wenwen Gao, Lin Lei, Xiliang Du and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Microbiome, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Chenxu Li *State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, Key Laboratory for Zoonosis Research of the Ministry of Education, Institute of Zoonosis, and, College of Veterinary Medicine , Jilin University, Changchun, 130062, China.
Guowen Liu *State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, Key Laboratory for Zoonosis Research of the Ministry of Education, Institute of Zoonosis, and, College of Veterinary Medicine , Jilin University, Changchun, 130062, China.
Yuting YangState Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, Key Laboratory for Zoonosis Research of the Ministry of Education, Institute of Zoonosis, and, College of Veterinary Medicine , Jilin University, Changchun, 130062, China.
Zhaoxin ShiState Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, Key Laboratory for Zoonosis Research of the Ministry of Education, Institute of Zoonosis, and, College of Veterinary Medicine , Jilin University, Changchun, 130062, China.
Qi ShaoState Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, Key Laboratory for Zoonosis Research of the Ministry of Education, Institute of Zoonosis, and, College of Veterinary Medicine , Jilin University, Changchun, 130062, China.
Zhiyuan FangState Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, Key Laboratory for Zoonosis Research of the Ministry of Education, Institute of Zoonosis, and, College of Veterinary Medicine , Jilin University, Changchun, 130062, China.
Yuxiang SongState Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, Key Laboratory for Zoonosis Research of the Ministry of Education, Institute of Zoonosis, and, College of Veterinary Medicine , Jilin University, Changchun, 130062, China.
Wenwen GaoState Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, Key Laboratory for Zoonosis Research of the Ministry of Education, Institute of Zoonosis, and, College of Veterinary Medicine , Jilin University, Changchun, 130062, China.
Lin LeiState Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, Key Laboratory for Zoonosis Research of the Ministry of Education, Institute of Zoonosis, and, College of Veterinary Medicine , Jilin University, Changchun, 130062, China.
Xiliang DuState Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, Key Laboratory for Zoonosis Research of the Ministry of Education, Institute of Zoonosis, and, College of Veterinary Medicine , Jilin University, Changchun, 130062, China. duxiliang@jlu.edu.cn.
Xinwei LiState Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, Key Laboratory for Zoonosis Research of the Ministry of Education, Institute of Zoonosis, and, College of Veterinary Medicine , Jilin University, Changchun, 130062, China. lixinwei100@126.com.

Funding

Graduate Innovation Fund of Jilin University 2024CX145National Key R&D Program of China 2023YFD1801400National Natural Science Foundation of China 32473105National Natural Science Foundation of China U24A20454Science Foundation of Education Department of Jilin Province JJKH20250156BS
6 · The paper itself

Abstract

backgroundPrepartum obesity predisposes dairy cows to a higher risk of postpartum metabolic disorder. Volatile fatty acids (VFA) produced through ruminal microbial fermentation of feed substrates serve as a key form of energy for dairy cows. However, the precise mechanisms through which the rumen microbiota promote adipocyte lipid accumulation in obese dairy cows remain to be elucidated. Thus, the aim of this study was to investigate the mechanisms by which rumen microbiota regulates prepartum obesity in dairy cows.

resultsPlasma glucose, insulin, triglyceride, and free fatty acids were greater in obese dairy cows. In the adipose tissue, the triglyceride content and expression of genes involved in lipid synthesis were higher in obese dairy cows. In the liver, the expression of genes involved in gluconeogenesis and lipid synthesis was higher in obese dairy cows. The ruminal total VFA, acetate, and propionate were higher in obese dairy cows compared to normal cows. The 16S rRNA gene analysis revealed that rumen bacteria, including Tidjanibacter inops_A, Rikenella massiliensis, Papillibacter cinnamivorans, and Parabacteroides merdae, were enriched in the rumen of obese dairy cows. Enrichment of these bacteria promoted carbohydrate degradation and VFA production. The metabolome analysis showed that obese dairy cows had elevated citric acid level in the rumen, which was positively associated with body condition score, body weight, adipocyte diameter, ruminal VFA concentration, and the abundance of VFA-producing bacteria.

conclusionsOur results suggest that rumen bacterial flora in prepartum obese dairy cows supply more VFA to the host, which may induce lipid deposition in adipocytes. Video Abstract.

Indexed as

Adipose TissueBacteriaGastrointestinal MicrobiomeLipogenesisObesityRumenAnimalsBlood GlucoseCattleFatty Acids, VolatileFemalePregnancyRNA, Ribosomal, 16STriglyceridesBlood GlucoseFatty Acids, VolatileRNA, Ribosomal, 16STriglyceridesDairy cowsGlucose and lipid metabolismPrepartum obesityRumen bacterial floraVolatile fatty acids

Identifiers

PMID41645262
PMCPMC12895798

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.