Evidence map›Paper›PMID 41645258›Full record

ArticleBMC veterinary research2026

Kruppel like factor 12 gene regulates ovarian granulosa cells proliferation and apoptosis via the PI3K/Akt signaling pathway.

Jiawei Cai, Bohao Zhao, Zhiyuan Bao, Yunpeng Li, Xiaoman Han, Yang Chen, Xinsheng Wu

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In one paragraph

Article in BMC veterinary research, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

7 authors.

Jiawei CaiCollege of Animal Science and Technology, Yangzhou University, Yangzhou, Jiangsu, 225009, China.
Bohao ZhaoCollege of Animal Science and Technology, Yangzhou University, Yangzhou, Jiangsu, 225009, China.
Zhiyuan BaoCollege of Animal Science and Technology, Yangzhou University, Yangzhou, Jiangsu, 225009, China.
Yunpeng LiCollege of Animal Science and Technology, Yangzhou University, Yangzhou, Jiangsu, 225009, China.
Xiaoman HanCollege of Animal Science and Technology, Yangzhou University, Yangzhou, Jiangsu, 225009, China.
Yang ChenCollege of Animal Science and Technology, Yangzhou University, Yangzhou, Jiangsu, 225009, China.
Xinsheng WuCollege of Animal Science and Technology, Yangzhou University, Yangzhou, Jiangsu, 225009, China. xswu@yzu.edu.cn.

Funding

China Agriculture Research System of MOF and MARA CARS-43-A-1
6 · The paper itself

Abstract

Granulosa cells (GCs), as the largest proportion of somatic cells in mammalian ovarian follicles, play a central regulatory role in follicular development and oocyte maturation. In-depth analysis of the molecular mechanisms related to the growth and development and functional regulation of GCs is of great significance for improving the regulatory network mechanism in the field of female reproduction. Kruppel Like Factor 12 (KLF12) gene is involved in the regulation of various cellular physiological activities, but its specific regulatory function in ovarian GCs is not yet clear.In this study, the coding sequence (CDS) of KLF12 gene in New Zealand female rabbits was cloned for the first time, and the expression abundance and cellular localization of this gene in ovarian tissues of female rabbits at different reproductive stages were systematically analyzed. The results showed that KLF12 was highly expressed in the ovaries of 18-month-old female rabbits. Functional studies found that overexpression of KLF12 significantly inhibited the synthesis of Estradiol (E2) and Progesterone (P) in GCs, and the expression of genes related to cell proliferation, apoptosis and cycle development also changed. Further Western blot (WB) detection confirmed that KLF12 overexpression significantly reduced the phosphorylation levels of key molecules PI3K and Akt in the PI3K/Akt signaling pathway, while KLF12 knockdown had the opposite effect on GCs function. These results reveal the molecular mechanism of KLF12 regulating the development and function of ovarian GCs in New Zealand female rabbits through PI3K/Akt signaling pathway, which not only provides a theoretical basis for improving the reproductive performance of female rabbits, but also provides a new scientific perspective for the study of the molecular mechanism of mammalian reproductive regulation.

Indexed as

ApoptosisGranulosa CellsKruppel-Like Transcription FactorsAnimalsCell ProliferationFemaleGene Expression RegulationPhosphatidylinositol 3-KinasesProto-Oncogene Proteins c-aktRabbitsSignal TransductionKruppel-Like Transcription FactorsPhosphatidylinositol 3-KinasesProto-Oncogene Proteins c-aktGranulosa cells proliferationKLF12 geneOvarian developmentPI3K/Akt signaling pathway

Identifiers

PMID41645258
PMCPMC12969923

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