Advancements in somatic mutation detection through loop-mediated isothermal amplification (LAMP) in human tumors.

Loop-mediated isothermal amplification (LAMP) is a robust and specific isothermal nucleic acid assessment technique. Due to its practicality and diverse detection strategies, LAMP has gained new insights in the analysis of somatic mutations in various tumor types, where the proportion of mutated DNA is often markedly low compared to wild-type DNA. We conducted an evaluation of articles from PubMed and Google Scholar published up to May 2025, focusing on somatic mutation detection in human cancers using LAMP. In addition to reviewing the articles found using specific keywords, we also examined and evaluated the references cited in those articles. By employing various primer design strategies specific to single base substitutions such as mismatch and loop primers, the researchers addressed the challenge of distinguishing between wild-type and mutant alleles. Furthermore, the specificity and sensitivity of the LAMP assay were enhanced by incorporating Locked Nucleic Acid (LNA) and Peptide Nucleic Acid (PNA) probes. The use of additives and adjustments in reaction conditions has also been discussed as strategies to improve reactions efficiency. LAMP demonstrates the capability to detect mutations present at levels as low as 0.1% in tumor tissues, indicating its potential for high-sensitivity mutation detection. The studies contribute to refining LAMP as a reliable, rapid, and cost-effective method for somatic mutation detection. The versatile detection approaches further enhance the utility and accessibility of the LAMP technique in various research and clinical settings. This paves the way for its broader application in clinical diagnostics and personalized medicine.