Evidence map›Paper›PMID 41644834›Full record

ArticleAnalytical and bioanalytical chemistry2026

An AuPt nanozyme-assisted CRISPR/Cas12a system for visual nucleic acid detection of pathogens.

Chenfei Zhao, Hangyu Guo, Jianai Chen, Yunyun Tan, Zhina Wu, Yaqin Zhang, Yingchun Li, Jiasi Wang, Rui Wang, He Zhang and 1 more

Abstract read
PubMed Publisher
In one paragraph

Article in Analytical and bioanalytical chemistry, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. The StABI5-Biology · 2026
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Chenfei Zhao *School of Life Sciences, Jilin University, Changchun, 130012, Jilin, China.
Hangyu Guo *School of Life Sciences, Jilin University, Changchun, 130012, Jilin, China.
Jianai ChenSchool of Life Sciences, Jilin University, Changchun, 130012, Jilin, China.
Yunyun TanEngineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, Jilin Agricultural University, Changchun, 130118, Jilin, China.
Zhina WuDepartment of Orthodontics, Hospital of Stomatology, Jilin University, Changchun, 130012, China.
Yaqin ZhangSchool of Life Sciences, Jilin University, Changchun, 130012, Jilin, China.
Yingchun LiSchool of Life Sciences, Jilin University, Changchun, 130012, Jilin, China.
Jiasi WangGuangdong Provincial Key Laboratory of Sensor Technology and Biomedical Instrument, School of Biomedical Engineering, Shenzhen Campus of Sun Yat-Sen University, Shenzhen, 518107, China.
Rui WangDepartment of Physics and Astronomy, University of Manchester, Manchester, M13 9PL, UK. rui.wang-4@manchester.ac.uk.
He ZhangChangchun Central Hospital, Changchun, 130012, China. 877053270@qq.com.
Di WangSchool of Life Sciences, Jilin University, Changchun, 130012, Jilin, China. jluwangdi@jlu.edu.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Potato early blight, caused by Alternaria solani, presents a significant threat to the potato industry. Existing detection methods for A. solani often fail to simultaneously achieve simplicity and accuracy. A gold-platinum (AuPt) nanozyme-assisted CRISPR/Cas12a system, termed the nanoparticle enzyme-assisted CRISPR detection (NACD assay) was developed. By integrating the precise target recognition of CRISPR with the enzyme-like activity of AuPt nanozymes, this system achieves simple, sensitive, and visual detection of A. solani. The NACD assay provided visual results through a distinct color change produced by the substrate catalyzed by the AuPt nanozyme. It can detect 100 copies/μL of the target dsDNA (A. solani 5.8S rRNA gene) and 10⁻

Indexed as

AlternariaCRISPR-Cas SystemsDNA, FungalGoldMetal NanoparticlesPlant DiseasesPlatinumBiosensing TechniquesSolanum tuberosumDNA, FungalGoldPlatinumAlternaria solaniCRISPR/Cas12aNanozymePaper-based biosensorVisualization

Identifiers

PMID41644834

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.