Evidence map›Paper›PMID 41644651›Full record

ArticleScientific reports2026

Preheating colistimethate sodium enhances its efficacy against multi/extensively drug-resistant Gram-negative bacilli in preservation fluid.

Daqian Tang, Lijie Zhang, Yazhe Duan, Yanhua Li, Yuhong Li, Pei Zhang, Yuxiang Wan, Kang Wu, Wenyu Zhao, Junhao Yu and 2 more

Abstract read
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In one paragraph

Article in Scientific reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Daqian Tang *Department of Organ Transplantation, Shanghai Changhai Hospital, The First Affiliated Hospital of Naval Medical University, Shanghai, China.
Lijie Zhang *Department of Organ Transplantation, Shanghai Changhai Hospital, The First Affiliated Hospital of Naval Medical University, Shanghai, China.
Yazhe DuanDepartment of Organ Transplantation, Shanghai Changhai Hospital, The First Affiliated Hospital of Naval Medical University, Shanghai, China.
Yanhua LiDepartment of Organ Transplantation, Shanghai Changhai Hospital, The First Affiliated Hospital of Naval Medical University, Shanghai, China.
Yuhong LiDepartment of Organ Transplantation, Shanghai Changhai Hospital, The First Affiliated Hospital of Naval Medical University, Shanghai, China.
Pei ZhangDepartment of Organ Transplantation, Shanghai Changhai Hospital, The First Affiliated Hospital of Naval Medical University, Shanghai, China.
Yuxiang WanClinical Laboratory, Shanghai Changhai Hospital, The First Affiliated Hospital of Naval Medical University, Shanghai, China.
Kang WuClinical Laboratory, Shanghai Changhai Hospital, The First Affiliated Hospital of Naval Medical University, Shanghai, China.
Wenyu ZhaoDepartment of Organ Transplantation, Shanghai Changhai Hospital, The First Affiliated Hospital of Naval Medical University, Shanghai, China.
Junhao YuDepartment of Organ Transplantation, Shanghai Changhai Hospital, The First Affiliated Hospital of Naval Medical University, Shanghai, China.
Li ZengDepartment of Organ Transplantation, Shanghai Changhai Hospital, The First Affiliated Hospital of Naval Medical University, Shanghai, China. shchyysyz@smmu.edu.cn.
Mingxing SuiDepartment of Organ Transplantation, Shanghai Changhai Hospital, The First Affiliated Hospital of Naval Medical University, Shanghai, China. mingxingsui@smmu.edu.cn.

Funding

National Natural Science Foundation of China No.81700548
6 · The paper itself

Abstract

This study aimed to evaluate the efficacy and preliminary safety of preheated colistimethate sodium (CMS) in reducing the load of multidrug-resistant and extensively drug-resistant (MDR/XDR) Gram-negative bacilli in preservation fluid (PF) under simulated static cold storage (SCS) conditions in vitro. CMS was preheated at 37-60 °C for 5 ~ 30 min. The antibacterial effects of preheated CMS were evaluated against five MDR/XDR strains: carbapenem-resistant Pseudomonas aeruginosa (CRPA), Klebsiella pneumoniae (CRKP), Escherichia coli (CREC), Acinetobacter baumannii (CRAB), and methicillin-resistant Staphylococcus aureus (MRSA). Bacterial counts were assessed after 3 h of SCS, and the average inhibition rate (AIR) was calculated. The safety experiment was performed to evaluate the nephrotoxicity of preheated CMS under hypothermic condition. Preheating CMS at 60 °C for 15 ~ 30 min significantly reduced bacterial loads of CRPA, CRKP, CREC, and CRAB, with average inhibition rates (AIR) up to 100%, 96.91%, 98.36%, and 85.06%, respectively. At 37 °C, extended heating (30 min) was required for partial efficacy against CRKP and CRAB, while CREC remained largely unaffected. MRSA showed no susceptibility to preheated CMS. Preheated CMS did not cause significant morphological alterations or reduction in HK-2 cell viability within 3 ~ 6 h of SCS. Thermal pretreatment of CMS at 60 °C represents a novel, practical, and safe strategy for PF decontamination, providing rapid bactericidal activity against frequently encountered MDR/XDR Gram-negative bacilli during the SCS process.

Indexed as

Anti-Bacterial AgentsColistinDrug Resistance, Multiple, BacterialGram-Negative BacteriaAcinetobacter baumanniiHumansMicrobial Sensitivity TestsAnti-Bacterial AgentsColistincolistinmethanesulfonic acidColistimethate sodiumDecontaminationExtensively drug-resistantGram-negative bacteriaMultidrug resistantOrgan transplantationPreservation fluidStatic cold storage

Identifiers

PMID41644651
PMCPMC12932831

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