Evidence map›Paper›PMID 41643681›Full record

ArticleCell reports methods2026

Multi-omic analysis of guided and unguided forebrain organoids reveals differences in cellular composition and metabolic profiles.

Marie Sejberg Øhlenschlæger, Pia Jensen, Jesper Foged Havelund, Sissel Ida Schmidt, Fadumo Abdullahi Mohamed, Magdalena Sutcliffe, Sofie Blomberg Elmkvist, Lucrezia Criscuolo, Steven W Wingett, Ilaria Chiaradia and 10 more

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Article in Cell reports methods, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

20 authors.

Marie Sejberg ØhlenschlægerDepartment of Biochemistry and Molecular Biology, University of Southern Denmark, 5230 Odense M, Denmark.
Pia JensenDepartment of Biochemistry and Molecular Biology, University of Southern Denmark, 5230 Odense M, Denmark.
Jesper Foged HavelundDepartment of Biochemistry and Molecular Biology, University of Southern Denmark, 5230 Odense M, Denmark.
Sissel Ida SchmidtDepartment of Biochemistry and Molecular Biology, University of Southern Denmark, 5230 Odense M, Denmark.
Fadumo Abdullahi MohamedDepartment for Veterinary and Animal Science, Faculty of Health and Medical Sciences, University of Copenhagen, 1870 Frederiksberg C, Denmark.
Magdalena SutcliffeMRC Laboratory of Molecular Biology, Cambridge Biomedical Campus, Cambridge CB2 0QH, UK.
Sofie Blomberg ElmkvistDepartment of Biochemistry and Molecular Biology, University of Southern Denmark, 5230 Odense M, Denmark.
Lucrezia CriscuoloDepartment of Biochemistry and Molecular Biology, University of Southern Denmark, 5230 Odense M, Denmark.
Steven W WingettMRC Laboratory of Molecular Biology, Cambridge Biomedical Campus, Cambridge CB2 0QH, UK.
Ilaria ChiaradiaMRC Laboratory of Molecular Biology, Cambridge Biomedical Campus, Cambridge CB2 0QH, UK.
Elif BayramDepartment of Clinical Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, 2200 Copenhagen N, Denmark; Copenhagen Research Centre for Biological and Precision Psychiatry, Mental Health Centre Copenhagen, Copenhagen University Hospital, 2900 Hellerup, Denmark.
Jeppe Allen Abildsten NicolaisenDepartment of Biochemistry and Molecular Biology, University of Southern Denmark, 5230 Odense M, Denmark.
Lene Andrup JakobsenDepartment of Biochemistry and Molecular Biology, University of Southern Denmark, 5230 Odense M, Denmark.
Jonathan BrewerDaMBIC, Department of Biochemistry and Molecular Biology, University of Southern Denmark, 5230 Odense M, Denmark.
Michael Eriksen BenrosDepartment of Clinical Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, 2200 Copenhagen N, Denmark; Copenhagen Research Centre for Biological and Precision Psychiatry, Mental Health Centre Copenhagen, Copenhagen University Hospital, 2900 Hellerup, Denmark.
Kristine FreudeDepartment for Veterinary and Animal Science, Faculty of Health and Medical Sciences, University of Copenhagen, 1870 Frederiksberg C, Denmark.
Nils Joakim FærgemanDepartment of Biochemistry and Molecular Biology, University of Southern Denmark, 5230 Odense M, Denmark.
Madeline A LancasterMRC Laboratory of Molecular Biology, Cambridge Biomedical Campus, Cambridge CB2 0QH, UK.
Martin Røssel LarsenDepartment of Biochemistry and Molecular Biology, University of Southern Denmark, 5230 Odense M, Denmark.
Helle BogetofteDepartment of Biochemistry and Molecular Biology, University of Southern Denmark, 5230 Odense M, Denmark. Electronic address: hbogetofte@bmb.sdu.dk.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Neural organoids are invaluable model systems for studying neurodevelopment, generated by either guided or unguided approaches. Despite the importance for the field, the resulting differences between these models are unclear. To obtain an unbiased comparison, we performed a multi-omic analysis of forebrain organoids generated in parallel with two widely applied guided and unguided protocols. The guided forebrain organoids contained a larger proportion of neurons, including GABAergic interneurons, whereas the unguided organoids contained significantly more choroid plexus, radial glia, and astrocytes at later stages. Substantial differences in metabolic profiles were identified, pointing to increased levels of oxidative phosphorylation and fatty acid β-oxidation in the unguided forebrain organoids and a higher reliance on glycolysis in the guided forebrain organoids. Overall, our study comprises a thorough description of the multi-omic differences between these guided and unguided forebrain organoids and provides an important resource for the neural organoid field studying neurodevelopment and disease.

Indexed as

MetabolomeOrganoidsProsencephalonAnimalsHumansMultiomicsNeurodevelopmentbrain organoidscerebral organoidsCP: neuroscienceCP: stem cellforebrainneural organoidspost-translational modificationsproteomicsschizophreniasingle-cell transcriptomics

Identifiers

PMID41643681
PMCPMC12946753

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.