Evidence map›Paper›PMID 41642882›Full record

ArticleACS synthetic biology2026

Slowpoke: An Automated Golden Gate Cloning Workflow for Opentrons OT-2 and Flex.

Koray Malcı, Fankang Meng, Henri Galez, Alicia Franja Da Silva, Joaquin Caro-Astorga, Gregory Batt, Tom Ellis

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in ACS synthetic biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Koray MalcıDepartment of Bioengineering, Imperial College London, London SW7 2AZ, U.K.
Fankang MengDepartment of Bioengineering, Imperial College London, London SW7 2AZ, U.K.
Henri GalezInstitut Pasteur, Inria, Université Paris Cité, 75015 Paris, France.
Alicia Franja Da SilvaInstitut Pasteur, Inria, Université Paris Cité, 75015 Paris, France.
Joaquin Caro-AstorgaDepartment of Bioengineering, Imperial College London, London SW7 2AZ, U.K.
Gregory BattInstitut Pasteur, Inria, Université Paris Cité, 75015 Paris, France.ORCID 0000-0003-1697-139X
Tom EllisDepartment of Bioengineering, Imperial College London, London SW7 2AZ, U.K.ORCID 0000-0001-5392-976X

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In synthetic biology, DNA assembly is a routine process where increasing demands for standardization, high-throughput capacity, and error-free execution are driving the development of accessible, automated solutions. Here, we present Slowpoke, a user-friendly and flexible workflow for Golden Gate-based cloning designed for the popular entry-cost, open-source liquid-handling platforms Opentrons OT-2 and Flex. Slowpoke automates the key steps of the DNA assembly process, including cloning,

Indexed as

Cloning, MolecularSoftwareSynthetic BiologyDNAEscherichia coliUser-Computer InterfaceWorkflowDNAautomationgenetic toolkitsGolden Gate cloninghigh-throughput DNA assemblystandardizationsynthetic biology

Identifiers

PMID41642882
PMCPMC12930513

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.