ArticleMethods in molecular biology (Clifton, N.J.)2026
Blue Native-PAGE Assay of NLR Protein Complexes Combined with Genetic Complementation in Nicotiana benthamiana.
Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
BN-PAGE (Blue Native-Polyacrylamide Gel Electrophoresis) is a non-denaturing electrophoretic technique used to analyze the molecular weight and oligomeric states of protein complexes under near-native conditions. NLRs (nucleotide-binding leucine-rich repeat proteins), which function as intracellular immune receptors in plants, form oligomeric higher-order complexes known as resistosomes upon activation by recognition of pathogen effectors-a mechanism elucidated through BN-PAGE and structural analyses. Here, we describe a method combining BN-PAGE with Agrobacterium-mediated complementation assay to investigate the resistosome formation of the NLR protein ZAR1 in Nicotiana benthamiana.
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