Lumi-Map, a Luciferase Bioluminescence Screen of Mutants Combined with MutMap Allows Rapid Dissection of Plant Immune Signaling Pathways.

Hiroaki Kato, Motoki Shimizu, Akira Abe, Ryohei Terauchi

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Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 · Who and what money

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4 authors.

Hiroaki KatoLaboratory of Crop Evolution, Graduate School of Agriculture, Kyoto University, Kyoto, Japan. kato.hiroaki.6a@kyoto-u.ac.jp.

Motoki ShimizuIwate Biotechnology Research Center, Kitakami, Japan.

Akira AbeIwate Biotechnology Research Center, Kitakami, Japan.

Ryohei TerauchiLaboratory of Crop Evolution, Graduate School of Agriculture, Kyoto University, Kyoto, Japan.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Genetic screening of mutants and identification of their causative genes are effective strategies for understanding plant signaling pathways. Here, we present Lumi-Map, a method that combines real-time luciferase bioluminescence monitoring with MutMap analysis to identify single nucleotide polymorphisms (SNPs) responsible for the mutant phenotypes. Real-time monitoring of reporter gene expression enables high-throughput screen of mutants with altered transcriptional responses. Once candidate mutants are isolated, a whole-genome sequencing-based method MutMap allows for rapid identification of the causative SNPs. Using reporter lines driven by defense-related gene promoters, Lumi-Map successfully identified genes involved in pattern-triggered immunity, including a novel receptor gene for pathogen-associated molecular patterns. By selecting appropriate luciferase reporter plants, Lumi-Map is expected to serve as a versatile platform for dissecting diverse signal transduction pathways in plants.

Indexed as

ArabidopsisLuciferasesLuminescent MeasurementsMutationPlant ImmunitySignal TransductionGene Expression Regulation, PlantGenes, ReporterPolymorphism, Single NucleotideLuciferasesGenetic screeningLuciferase bioluminescence monitoringLumi-MapSignal transduction pathwaysWhole-genome sequencing

Identifiers

PMID41642512

DOI10.1038/s41467-020-18782-1

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.