Evidence map›Paper›PMID 41642456›Full record

ArticleAnnals of biomedical engineering2026

Mechanical Stretch Disrupts Calcium Dynamics and Redistributes Piezo1 in Human Astrocytes.

Shahrzad Shiravi, Akash Chakka, Xi Xiao, Meilin Fernandez Garcia, Alexandra Yufa, Angela Mitevska, Carina Seah, Huanyao Gao, Laura M Huckins, Kristen J Brennand and 1 more

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In one paragraph

Article in Annals of biomedical engineering, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Review
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

11 authors.

Shahrzad Shiravi *Department of Mechanical and Industrial Engineering, University of Illinois Chicago, Chicago, IL, 60607, USA.
Akash Chakka *Department of Psychiatry, Yale University School of Medicine, 34 Park Street, New Haven, CT, 06520, USA.
Xi Xiao *Department of Psychiatry, Yale University School of Medicine, 34 Park Street, New Haven, CT, 06520, USA.
Meilin Fernandez Garcia *Department of Psychiatry, Yale University School of Medicine, 34 Park Street, New Haven, CT, 06520, USA.
Alexandra YufaDepartment of Biomedical Engineering, University of Illinois Chicago, Chicago, IL, 60607, USA.
Angela MitevskaDepartment of Biomedical Engineering, University of Illinois Chicago, Chicago, IL, 60607, USA.
Carina SeahDepartment of Psychiatry, Yale University School of Medicine, 34 Park Street, New Haven, CT, 06520, USA.
Huanyao GaoDepartment of Psychiatry, Yale University School of Medicine, 34 Park Street, New Haven, CT, 06520, USA.
Laura M HuckinsDepartment of Psychiatry, Yale University School of Medicine, 34 Park Street, New Haven, CT, 06520, USA.
Kristen J BrennandDepartment of Psychiatry, Yale University School of Medicine, 34 Park Street, New Haven, CT, 06520, USA.
John D FinanDepartment of Mechanical and Industrial Engineering, University of Illinois Chicago, Chicago, IL, 60607, USA. jdfinan@uic.edu.ORCID http://orcid.org/0000-0003-4626-9702

Funding

Applying human in vitro models to understand the link between trauma and tau pathologyR21NS135179 · NINDS · UNIVERSITY OF ILLINOIS AT CHICAGO · PI FINAN, JOHN D · 2023 to 2023
$457k
NINDS NIH HHS R21NS135179
6 · The paper itself

Abstract

purposeAstrocytes regulate the activity of nearby neurons so disruption of astrocyte calcium dynamics by traumatic brain injury (TBI) could have profound consequences for neural network activity in the brain. This study aimed to define how mechanical stretch injury alters calcium signaling, mitochondrial membrane potential, and mechanosensitive ion channel organization in human induced pluripotent stem cell (hiPSC)-derived astrocytes.

methodsHuman iPSC-derived astrocytes were subjected to controlled two-dimensional stretch injury across multiple severities. Live-cell calcium and mitochondrial membrane potential imaging, Piezo1 immunostaining, and RNA sequencing were used to assess functional and transcriptional responses.

resultsCell viability, mitochondrial membrane potential, and spontaneous calcium transients declined in a severity-dependent manner. At moderate injury levels, reductions in mitochondrial function, calcium dynamics, and Piezo1 spatial distribution were transient. RNA sequencing identified 196 differentially expressed genes, including downregulation of mitochondrial and oxidative metabolic pathways and upregulation of cortical thinning-associated pathways.

conclusionThis platform captures functional and molecular hallmarks of astrocyte injury and provides a human in vitro model for studying mechanobiological pathways linking TBI to neurodegenerative processes.

Indexed as

AstrocytesCalciumCalcium SignalingIon ChannelsStress, PhysiologicalBrain Injuries, TraumaticCell DifferentiationCells, CulturedCell SurvivalGene Expression ProfilingGene Expression RegulationHumansInduced Pluripotent Stem CellsIntravital MicroscopyMembrane Potential, MitochondrialMicroscopy, ConfocalCalciumIon ChannelsPIEZO1 protein, humanCalcium dynamicshiPSC-derived astrocytesMitochondrial dysfunctionPiezo1RNA sequencingTraumatic brain injury

Identifiers

PMID41642456
PMCPMC13186902

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.