ArticleBiotechnology letters2026
Optimizing Lactiplantibacillus plantarum JCM 1149 growth conditions for improvement of the gelation and nutritional qualities of fermented soy milk.
Article in Biotechnology letters, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Exopolysaccharide (EPS) is a biopolymer produced by microorganisms. This study aimed to optimize EPS production from lactic acid bacteria (LAB) to enhance the gelation quality of fermented soy milk by varying physical growth conditions. EPS was purified using gel filtration column chromatography, and the proximate composition of the fermented soy milk was analyzed according to AOAC methods. Lactiplantibacillus plantarum JCM 1149 produced a crude EPS yield of 166.4 mg/100 mL under optimized conditions (i.e. 35 °C, pH 4.8, 0.5 mL inoculum) after 24 h, while the purified EPS was 158.6 mg/100 mL with a glucose equivalent of 0.209 g/100 mL. Fermented soy milk produced under these conditions (sample T) was compared to a control prepared with a commercial yogurt starter culture (sample C). Sample T had a higher water-holding capacity (69.1%) and lower syneresis (35.80%) than sample C (30.80% and 41.01%, respectively). No significant differences (p > 0.05) were observed between samples for protein, lipid, nitrogen-free extract, or fiber content. However, sample T showed significantly higher moisture content (80.62 ± 0.30%) and energy (46.64 ± 0.29 kcal) slightly lower ash content (0.37 ± 0.00%) compared to sample C (0.41 ± 0.00%). Total viable counts were similar between samples C (7.10 × 10
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