[Design and synthesis of molecularly imprinted polymers and their applications in medical diagnosis and biocatalysis].

Molecularly imprinted technology （MIT） represents an advanced synthetic strategy that emulates biological recognition mechanisms， such as antigen-antibody or enzyme-substrate interactions， by creating three-dimensional cavity-like structures through the directional assembly of functional monomers around a template molecule. This process generates spatial and functional complementarity， enabling highly selective recognition of target species. Molecularly imprinted polymers （MIPs）， often described as "synthetic antibodies"， overcome the intrinsic limitations of natural biomolecules by offering superior selectivity， robustness， cost-effectiveness， and structural tunability. These features position MIPs as promising alternatives to natural antibodies in targeted sensing and drug delivery， with broad applications across biomedical， environmental， and pharmaceutical domains， including pollutant detection， and food safety monitoring. Despite substantial progress， key challenges remain， such as uneven imprinting layers， template residue， and limited aqueous compatibility in macromolecular imprinting. Furthermore， issues of industrial scalability， unclear recognition mechanisms， and insufficient integration with emerging fields such as microfluidics and artificial intelligence have hindered large-scale translation. In recent years， our research team has systematically advanced MIT through a tri-dimensional strategy encompassing high-throughput monomer screening， mechanistic elucidation of molecular recognition， and directional assembly of functional units. By establishing a standardized monomer library and integrating molecular dynamics simulations， we achieved precise material design under complex conditions. Through process optimization and material innovation， we developed a highly efficient solid-phase surface imprinting method that enables the fabrication of smart MIPs with stimuli-responsive properties （e.g.， temperature and pH）. These MIPs exhibit markedly enhanced binding affinity， with equilibrium dissociation constant （