Evidence map›Paper›PMID 41641275›Full record

ArticleFrontiers in immunology2025

Allergen extract outperforms molecular components in basophil activation test in a pediatric cohort.

Alexandre Chhing, Simone Choi, Dounia Khelifi-Touhami, Aïcha Abbas, Eric Ballot, Nathalie Cottel, Sarah Saf, Garance Germain, Nathalie Lambert, Pierre Challier and 6 more

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PubMed Full text at PMC Publisher
In one paragraph

Article in Frontiers in immunology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors.

Alexandre ChhingImmunology Department, St-Antoine & Trousseau Hospital, Sorbonne University, AP-HP, Paris, France.
Simone ChoiImmunology Department, St-Antoine & Trousseau Hospital, Sorbonne University, AP-HP, Paris, France.
Dounia Khelifi-TouhamiImmunology Department, St-Antoine & Trousseau Hospital, Sorbonne University, AP-HP, Paris, France.
Aïcha AbbasImmunology Department, St-Antoine & Trousseau Hospital, Sorbonne University, AP-HP, Paris, France.
Eric BallotImmunology Department, St-Antoine & Trousseau Hospital, Sorbonne University, AP-HP, Paris, France.
Nathalie CottelAllergology Department, Trousseau Hospital, Sorbonne University, AP-HP, Paris, France.
Sarah SafAllergology Department, Trousseau Hospital, Sorbonne University, AP-HP, Paris, France.
Garance GermainAllergology Department, Trousseau Hospital, Sorbonne University, AP-HP, Paris, France.
Nathalie LambertAllergology Department, Trousseau Hospital, Sorbonne University, AP-HP, Paris, France.
Pierre ChallierPaediatric Nutrition and Gastroenterology Department, Trousseau Hospital, Sorbonne University, AP-HP, Paris, France.
Sylvie PauliatPaediatric Nutrition and Gastroenterology Department, Trousseau Hospital, Sorbonne University, AP-HP, Paris, France.
Anaïs LemoinePaediatric Nutrition and Gastroenterology Department, Trousseau Hospital, Sorbonne University, AP-HP, Paris, France.
Melisande Bourgoin-HeckHealth Environmental Risk Assessment (HERA) Team, UMR261 MERIT, IRD, Inserm 1344, Faculté de Pharmacie, Université Paris Cité, Paris, France.
Jocelyne JustAllergology Department, Trousseau Hospital, Sorbonne University, AP-HP, Paris, France.
Stéphanie WaninHealth Environmental Risk Assessment (HERA) Team, UMR261 MERIT, IRD, Inserm 1344, Faculté de Pharmacie, Université Paris Cité, Paris, France.
Yannick ChantranImmunology Department, St-Antoine & Trousseau Hospital, Sorbonne University, AP-HP, Paris, France.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Background: The use of molecular allergens have greatly improved the clinical relevance of specific IgE serologies during allergy work-up. Very little is known regarding the added value of molecular allergens in the results of the basophil activation test (BAT). Objective: To study the BAT concordance when using similar amounts of molecular allergens against source extracts, and to assess factors associated with BAT discrepancies between extract and related component. Methods: Systematic retrospective monocentric study of all BAT performed at the Trousseau Hospital, Paris, France, with both molecular components and corresponding extract. Results: Data from 213 interpretable extract/component BAT pairs (89 cow milk, 70 wheat, 28 house dust mites (HDM), 12 peanut, 9 hen egg, 3 peach, 1 apple, 1 chicken meat), corresponding to 150 blood samples from 109 patients were analyzed. Among BAT pairs showing allergen sensitizations, the two reagents only showed moderate agreement (κ = 0.62), with 18% (36/201) discordant BAT results. Among 36 cases of discrepant BAT results between extract and molecular component, 69% (25/36) were extract Conclusion: In our study population, molecular components had lower capacity than the corresponding extract to activate IgE-sensitized basophils, which partly explain higher rates of true positive extract

Indexed as

AllergensBasophil Degranulation TestBasophilsHypersensitivityAdolescentAnimalsChildChild, PreschoolFemaleHumansImmunoglobulin EInfantMaleRetrospective StudiesAllergensImmunoglobulin Eallergy diagnosisbasophil activation testbiomarkersmethod comparisonmolecular allergens

Identifiers

PMID41641275
PMCPMC12864089

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.