ArticleFrontiers in cell and developmental biology2026
Single-cell RNA sequencing revealed cell heterogeneity in sagittal suture mesenchyme.
Article in Frontiers in cell and developmental biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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1 citing paper in PubMed.
- Advances in Research on Autophagy in Steroid-Induced Osteonecrosis of the Femoral Head: Dual Regulation of Its Beneficial and Detrimental Effects.Drug design, development and therapy · 2026Review
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7 authors.
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Abstract
Introduction: The formation and homeostatic maintenance of cranial sutures rely on cellular activities within the suture mesenchyme. However, how mesenchymal stem/stromal cells (MSCs) rapidly and extensively contribute to suture and cranial development remains insufficiently explored. Methods: We integrated 10x Genomics and Smart-seq3 single-cell transcriptomic sequencing to analyze cellular subpopulations in the sagittal suture mesenchyme. CytoTRACE2 analysis was performed to quantitatively assess the differentiation status of each cell population. We further characterized the progenitor with characteristics of transient amplifying cells (TACs) via 5-ethynyl-2'-deoxyuridine (EdU) assays, in situ hybridization, and lineage tracing using Results: Smart-seq3 showed higher sequencing depth and improved capture efficiency for target cell populations. Then, we identified a proliferative progenitor population in the sagittal suture that exhibited features of TACs. These TACs were a committed, proliferative direct lineage of suture mesenchymal stem/stromal cells (SuSCs) and responsible for rapid development of cranial structures. Additionally, Discussion: This study provides the first identification of TACs within the developing cranial suture niche and elucidates key regulatory genes and signaling networks in SuSCs and TACs, thereby providing a theoretical framework for understanding the mechanisms underlying cranial suture formation and homeostasis.
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