Evidence map›Paper›PMID 41640351›Full record

ArticleChembiochem : a European journal of chemical biology2026

Enhancement of Nitrogenase Activity in Recombinant Escherichia coli by Replacing Upstream Region of nif Operon from Paenibacillus Species.

Yuki Honda, Kotone Takeuchi, Sawako Hayakawa, Daisuke Koma, Hiroshi Fujii

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Chembiochem : a European journal of chemical biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Yuki HondaDepartment of Chemistry, Biology and Environmental Science, Faculty of Science, Nara Women's University, Nara, Japan.ORCID https://orcid.org/0000-0002-6342-1924
Kotone TakeuchiDepartment of Chemistry, Biology and Environmental Science, Faculty of Science, Nara Women's University, Nara, Japan.
Sawako HayakawaDepartment of Chemistry, Biology and Environmental Science, Faculty of Science, Nara Women's University, Nara, Japan.
Daisuke KomaResearch Division of Environmental Technology, Osaka Research Institute of Industrial Science and Technology, Osaka, Japan.ORCID https://orcid.org/0000-0002-8255-5872
Hiroshi FujiiDepartment of Chemistry, Biology and Environmental Science, Faculty of Science, Nara Women's University, Nara, Japan.ORCID https://orcid.org/0000-0003-4611-2983

Funding

Japan Society for the Promotion of Science JP24K01272
6 · The paper itself

Abstract

Recombinant Escherichia coli with nitrogen-fixation activity has been constructed using a simple approach of introducing a minimal gene set of nitrogen fixation (nif) genes along with its upstream region from Paenibacillus species; however, limitations may exist in nif expression. To understand the limitations of heterologous mass production of nitrogenase in E. coli, we evaluated this approach using various nif operons cloned from Paenibacillus species. Initial attempts revealed that, despite the high nitrogenase activity observed in the parental strains, the corresponding nif operons did not consistently function in E. coli. Further analysis revealed that certain upstream regions of the nif operons function as constitutive σ

Indexed as

Escherichia coliNitrogenaseOperonPaenibacillusNitrogen FixationPromoter Regions, GeneticNitrogenaseEscherichia coliheterologous expressionnitrogenasenitrogen fixationPaenibacillus species

Identifiers

PMID41640351
PMCPMC12873634

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.