Evidence map›Paper›PMID 41640095›Full record

ArticleThe FEBS journal2026

Selective targeting of cortactin tandem repeat acetylation by human lysine deacetylases.

Jan Komarek, Miroslava Vosahlikova, Zsofia Kutil, Zora Novakova, Julia Kudlacova, Ruzena Tuckova, Marat Meleshin, Barbora Havlinova, Pavlina Jaklova, Jana Ptackova and 3 more

Abstract read
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In one paragraph

Article in The FEBS journal, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Jan KomarekInstitute of Biotechnology of the Czech Academy of Sciences, BIOCEV, Vestec, Czech Republic.ORCID https://orcid.org/0000-0003-4496-1658
Miroslava VosahlikovaInstitute of Biotechnology of the Czech Academy of Sciences, BIOCEV, Vestec, Czech Republic.
Zsofia KutilInstitute of Biotechnology of the Czech Academy of Sciences, BIOCEV, Vestec, Czech Republic.
Zora NovakovaInstitute of Biotechnology of the Czech Academy of Sciences, BIOCEV, Vestec, Czech Republic.ORCID https://orcid.org/0000-0001-9804-6346
Julia KudlacovaInstitute of Biotechnology of the Czech Academy of Sciences, BIOCEV, Vestec, Czech Republic.
Ruzena TuckovaInstitute of Biotechnology of the Czech Academy of Sciences, BIOCEV, Vestec, Czech Republic.
Marat MeleshinDepartment of Enzymology, Charles Tanford Protein Center, Institute of Biochemistry and Biotechnology, Martin-Luther-University Halle-Wittenberg, Halle/Saale, Germany.
Barbora HavlinovaInstitute of Biotechnology of the Czech Academy of Sciences, BIOCEV, Vestec, Czech Republic.
Pavlina JaklovaInstitute of Biotechnology of the Czech Academy of Sciences, BIOCEV, Vestec, Czech Republic.
Jana PtackovaInstitute of Biotechnology of the Czech Academy of Sciences, BIOCEV, Vestec, Czech Republic.
Cordelia Schiene-FischerDepartment of Enzymology, Charles Tanford Protein Center, Institute of Biochemistry and Biotechnology, Martin-Luther-University Halle-Wittenberg, Halle/Saale, Germany.
Mike SchutkowskiDepartment of Enzymology, Charles Tanford Protein Center, Institute of Biochemistry and Biotechnology, Martin-Luther-University Halle-Wittenberg, Halle/Saale, Germany.
Cyril BarinkaInstitute of Biotechnology of the Czech Academy of Sciences, BIOCEV, Vestec, Czech Republic.ORCID https://orcid.org/0000-0003-2751-3060

Funding

Akademie Vd esk Republiky 86652036European Regional Development Fund CZ.02.01.01/00/23_015/0008175Grantov Agentura esk Republiky 2131806SMinistry of Education, Youth and Science LM2023042
6 · The paper itself

Abstract

Lysine acetylation within the tandem repeat region of cortactin (CTTN) regulates its actin-binding function and has been linked to cancer cell migration and neuronal development. While several lysine deacetylases (KDACs) have been implicated in modulating CTTN acetylation in cells, their site specificity and direct enzymatic roles remain poorly defined. Here, we use genetic code expansion to generate seven site-specifically acetylated CTTN variants and assess their deacetylation by human KDACs in a fully reconstituted in vitro system. Our results identify HDAC6 as the primary CTTN deacetylase, acting via its second catalytic domain (DD2), and demonstrate that SIRT1 and SIRT2 also directly deacetylate CTTN at overlapping sites in an NAD

Indexed as

CortactinHistone DeacetylasesLysineSirtuin 1Sirtuin 2AcetylationCatalytic DomainHistone Deacetylase 6HumansProtein Processing, Post-TranslationalRepressor ProteinsSubstrate SpecificityTandem Repeat SequencesCortactinCTTN protein, humanHDAC6 protein, humanHDAC8 protein, humanHistone Deacetylase 6Histone DeacetylasesLysineRepressor ProteinsSIRT1 protein, humanSIRT2 protein, humanSirtuin 1Sirtuin 2acetylationcortactingenetic code expansionhistone deacetylase (HDAC)sirtuinsubstrate specificity

Identifiers

PMID41640095
PMCPMC13278358

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.