ArticleNature communications2026
Branched-chain amino acid specialization drove diversification within Calditenuaceae (Caldarchaeia) and enables their cultivation.
Dengxun Lai, Damon Mosier, Marike Palmer, Xavier Mayali, Juliet Johnston, Walter Saldivar, Jonathan K Covington, Jian-Yu Jiao, Ranjani Murali, Cale O Seymour and 10 more
Abstract read
In one paragraphArticle in Nature communications, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
2 · The registryThe trial behind it
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3 · Its place in the literatureWho cites it
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4 · The recordCorrections and comments
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5 · Who and what moneyAuthors and funding
20 authors.
Xavier MayaliPhysical and Life Sciences Directorate, Lawrence Livermore National Lab, Livermore, CA, USA.ORCID 0000-0002-2170-0773 Juliet JohnstonPhysical and Life Sciences Directorate, Lawrence Livermore National Lab, Livermore, CA, USA.
Walter SaldivarDepartment of Biology, California State University, San Bernardino, CA, USA.
Jonathan K CovingtonSchool of Life Sciences, University of Nevada, Las Vegas, NV, USA.
Jian-Yu JiaoState Key Lab of Biocontrol, Guangdong Provincial Key Lab of Plant Resources, School of Life Sciences, Sun Yat-Sen University, Guangzhou, PR China.
Ranjani MuraliSchool of Life Sciences, University of Nevada, Las Vegas, NV, USA.
Cale O SeymourSchool of Life Sciences, University of Nevada, Las Vegas, NV, USA.
Lan LiuState Key Lab of Biocontrol, Guangdong Provincial Key Lab of Plant Resources, School of Life Sciences, Sun Yat-Sen University, Guangzhou, PR China.ORCID 0000-0001-8139-7246 Zheng-Shuang HuaDepartment of Environmental Science and Engineering, University of Science and Technology of China, Hefei, PR China.ORCID 0000-0002-2405-4228 Wen-Jun LiState Key Lab of Biocontrol, Guangdong Provincial Key Lab of Plant Resources, School of Life Sciences, Sun Yat-Sen University, Guangzhou, PR China.ORCID 0000-0002-1233-736X Peter K WeberPhysical and Life Sciences Directorate, Lawrence Livermore National Lab, Livermore, CA, USA.ORCID 0000-0001-6022-6050 Jennifer Pett-RidgePhysical and Life Sciences Directorate, Lawrence Livermore National Lab, Livermore, CA, USA.ORCID 0000-0002-4439-2398 Daniel R ColmanDepartment of Microbiology and Cell Biology, Montana State University, Bozeman, MT, USA.ORCID 0000-0002-3253-6833 Eric S BoydDepartment of Microbiology and Cell Biology, Montana State University, Bozeman, MT, USA.
Takuro NunouraResearch Center for Bioscience and Nanoscience (CeBN), Japan Agency for Marine-Earth Science and Technology (JAMSTEC), Yokosuka, Japan.ORCID 0000-0003-2323-0880 Jeremy A DodsworthDepartment of Biology, California State University, San Bernardino, CA, USA. jdodsworth@csusb.edu.ORCID 0000-0003-2014-5488 Brian P HedlundSchool of Life Sciences, University of Nevada, Las Vegas, NV, USA. brian.hedlund@unlv.edu.ORCID 0000-0001-8530-0448 Funding
National Aeronautics and Space Administration (NASA) 80NNSC17KO548National Aeronautics and Space Administration (NASA) 80NSSC19M0150National Aeronautics and Space Administration (NASA) 80NSSC25M0046National Natural Science Foundation of China (National Science Foundation of China) 32370011National Natural Science Foundation of China (National Science Foundation of China) 92251302National Science Foundation (NSF) 1557042National Science Foundation (NSF) 2038420
6 · The paper itselfAbstract
Many thermophiles that are abundant in high-temperature geothermal systems have never been cultivated and are poorly understood, including deeply branching members of the archaeal phylum Thermoproteota. Here, we describe the genome-guided cultivation of one such organism, Calditenuis ramacidaminiphagus, and show that it has evolved a heterotrophic metabolism focused on branched-chain amino acids (BCAAs). Initially, fluorescence in situ hybridization and nanoscale secondary ion mass spectrometry (FISH-nanoSIMS) showed that Cal. ramacidaminiphagus assimilated amino acids rapidly in casamino acid-amended enrichment cultures. Metagenome and metaproteome analyses showed a high abundance and expression of BCAA transporter genes, suggesting a BCAA-focused metabolism. This inference was supported by the subsequent enrichment of Cal. ramacidaminiphagus in BCAA-fed cultures, reaching 2.66×10
Indexed as
Amino Acids, Branched-ChainArchaeaIn Situ Hybridization, FluorescenceMetagenomePhylogenyAmino Acids, Branched-Chain
Identifiers
PMID41639055
PMCPMC12979851
What OpenQuestion holds
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