Evidence map›Paper›PMID 41638534›Full record

ArticleVirus research2026

Revelation of core promoter elements and bidirectional regulation of Geminivirus genes in Escherichia coli.

Hsiao-En Lin, Ying-Wen Huang, Yi-Chin Lai, Na-Sheng Lin, Yau-Heiu Hsu, Chung-Chi Hu

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In one paragraph

Article in Virus research, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Hsiao-En LinGraduate Institute of Biotechnology, National Chung Hsing University, Taichung 40227, Taiwan. Electronic address: n658932417@gmail.com.
Ying-Wen HuangGraduate Institute of Biotechnology, National Chung Hsing University, Taichung 40227, Taiwan; Advanced Plant Biotechnology Center, National Chung Hsing University, Taichung 40227, Taiwan.
Yi-Chin LaiGraduate Institute of Biotechnology, National Chung Hsing University, Taichung 40227, Taiwan.
Na-Sheng LinAdvanced Plant Biotechnology Center, National Chung Hsing University, Taichung 40227, Taiwan.
Yau-Heiu HsuGraduate Institute of Biotechnology, National Chung Hsing University, Taichung 40227, Taiwan; Advanced Plant Biotechnology Center, National Chung Hsing University, Taichung 40227, Taiwan.
Chung-Chi HuGraduate Institute of Biotechnology, National Chung Hsing University, Taichung 40227, Taiwan; Advanced Plant Biotechnology Center, National Chung Hsing University, Taichung 40227, Taiwan. Electronic address: cchu@dragon.nchu.edu.tw.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Several geminiviruses have been show to possess cross-kingdom gene expression capability. In addition, the intergenic region (IR) of geminivirus genomes is known to regulate the transcription of viral early genes (complementary-sense, C-sense) and late genes (virion-sense, V-sense) located in opposite directions in viral genomic DNAs. However, the underlying mechanism remained elusive. In this study, the transcriptional regulation activities in the IR of ageratum yellow vein virus isolate NT (AYVV-NT), a monopartite geminivirus, were characterized in Escherichia coli, by using a promoter-trapping system. A functional bidirectional promoter core and regulatory elements for C- and V-sense genes were identified in the IR of AYVV-NT. Quantitative analyses revealed differences in promoter activity in various E. coli strains, suggesting that promoter regulation is strain-dependent and influenced by bacterial transcription factors. These findings provide insights into how plant-infecting geminiviruses may regulate gene expression in prokaryotic environments and highlight the potential applications of such viral regulatory elements in bacterial expression systems.

Indexed as

Escherichia coliGeminiviridaeGene Expression Regulation, ViralPromoter Regions, GeneticDNA, IntergenicDNA, ViralTranscription, GeneticDNA, IntergenicDNA, ViralCross-kingdomGeminivirusesIntergenic region (IR)Prokaryotic systemsTranscriptionTranscription regulation

Identifiers

PMID41638534
PMCPMC12907893

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.