Evidence map›Paper›PMID 41638244›Full record

ArticleJournal of experimental botany2026

Stem nitrogen accumulation through vegetative storage proteins and mobilization to seeds supports high-yielding soybean.

Luzviminda A R Sazon, Patricio Grassini, Sophie Alvarez, Reka Howard, Juan Pablo Monzon, Gautam Sarath, Michael J Naldrett, John Lindquist, James Specht, Nicolas Cafaro La Menza

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of experimental botany, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

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5 · Who and what money

Authors and funding

10 authors.

Luzviminda A R SazonDepartment of Agronomy and Horticulture, University of Nebraska-Lincoln, PO Box 830915, Lincoln, NE 68583-0915, USA.
Patricio GrassiniDepartment of Agronomy and Horticulture, University of Nebraska-Lincoln, PO Box 830915, Lincoln, NE 68583-0915, USA.ORCID 0000-0002-7501-842X
Sophie AlvarezProteomics and Metabolomics Facility, Nebraska Center for Biotechnology, University of Nebraska-Lincoln, Lincoln, NE 68588, USA.ORCID 0000-0001-8550-2832
Reka HowardDepartment of Statistics, University of Nebraska-Lincoln, 340 Hardin Hall North Wing, Lincoln, NE 68583-0963, USA.ORCID 0000-0002-8837-3770
Juan Pablo MonzonNational Scientific and Science and Technical Research Council (CONICET), Department of Agricultural Science, National University of Mar del Plata, Integrated Unit Balcarce Route 226, km 73.5, CC 276, CP 7620, Balcarce, Buenos Aires, Argentina.
Gautam SarathWheat, Sorghum, and Forage Research Unit, United States Department of Agriculture-Agricultural Research Service (USDA-ARS), Lincoln, NE 68583, USA.ORCID 0000-0002-3145-9493
Michael J NaldrettProteomics and Metabolomics Facility, Nebraska Center for Biotechnology, University of Nebraska-Lincoln, Lincoln, NE 68588, USA.
John LindquistDepartment of Agronomy and Horticulture, University of Nebraska-Lincoln, PO Box 830915, Lincoln, NE 68583-0915, USA.ORCID 0000-0002-6293-7072
James SpechtDepartment of Agronomy and Horticulture, University of Nebraska-Lincoln, PO Box 830915, Lincoln, NE 68583-0915, USA.ORCID 0000-0003-1859-4858
Nicolas Cafaro La MenzaDepartment of Agronomy and Horticulture, University of Nebraska-Lincoln, PO Box 830915, Lincoln, NE 68583-0915, USA.ORCID 0000-0002-9982-854X

Funding

Agricultural Research Division - Institute of Agriculture and Natural Resources at the University of NebraskaNebraska Soybean Board
6 · The paper itself

Abstract

The role of stems as a source of nitrogen (N) for growing seeds has received little attention in soybean. This study evaluated N storage and mobilization from stems to seeds across 42 high-yielding environments under contrasting N supply: a 'zero-N' treatment, in which crops relied on soil and biological N fixation, and a 'full-N' treatment consisting of ample N fertilizer. We measured the accumulated N per organ before seed filling and subsequent N mobilization from vegetative organs to seeds. Vegetative storage protein (VSP) abundance in stems was quantified via proteomics. Yield ranged from 4.2 to 7.3 Mg ha-1, with +11% yield in full-N (6.1 Mg ha-1) compared with zero-N (5.5 Mg ha-1). The full-N treatment resulted in more mobilized N from stems (+42%) and leaves (+22%) to seeds than zero-N treatment, which was attributed to the larger amount of N stored in stems and leaves before seed filling (+37% and +21%, respectively). Among the 5335 identified proteins at the peak of N accumulation, VSPα (+1.4%) and VSPβ (+0.8%) showed the greatest increase in full-N. We conclude that N stored in stems as VSPs before seed filling plays a key role in meeting the seed N demand in high-yielding soybean crops.

Indexed as

Glycine maxNitrogenPlant ProteinsPlant StemsSeedsPlant LeavesNitrogenPlant ProteinsGlycoprotein 28 kDaglycoprotein 31 kDahigh yieldnitrogennitrogen mobilizationsoybeanstemvegetative storage proteins

Identifiers

PMID41638244
PMCPMC13187634

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.