ArticleJPhys photonics2026
Generalized 3D quasi-phase-matching model of image contrast in second harmonic generation microscopy of fibrillar collagen architectures.
Article in JPhys photonics, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Second harmonic generation (SHG) microscopy is a powerful tool in assessing collagen structure, especially with respect to differentiating the respective architectures of normal and diseased tissues. An under-explored area is exploiting SHG to determine the sub-resolution aspects of the collagen fibril size, polarity, and packing (∼50-100 nm diameters). Due to the phase-matching and associated coherence of SHG, these structural aspects are encoded in the wavelength dependence of the spatial emission and relative conversion efficiency, denoted the creation attributes. As a means to extract this information, we present a generalized 3D computational/theoretical treatment based on quasi-phase-matching (QPM), which can predict the SHG emission pattern and relative conversion efficiency using collagen models based on 3D biomimetic fibril architectures. Specifically, we incorporate random rather than purely periodic structures and non-ideal phase-matching
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