ArticleJOR spine2026
Advancement in the Methods for Isolating High Quality RNA From Mouse and Rat Intervertebral Disc.
Article in JOR spine, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
2 citing papers in PubMed.
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Background: Extracting high-quality RNA from intervertebral disc (IVD) tissues poses a great technical challenge due to the presence of a proteoglycan-rich extracellular matrix and naturally occurring RNases. Current study aims to build on previously reported RNA isolation methods to develop sample preparation and RNA precipitation methods that yield high RNA integrity from mouse and rat IVD tissue sub-types reproducibly. Methods: High salt-isopropanol RNA precipitation was tested on three sample types. (1) Freshly dissected mouse IVD tissues: nucleus pulposus (NP) and annulus fibrosus (AF) tissues from mouse lumbar and caudal IVD tissues were collected in Results: RNA isolated from freshly dissected mouse lumbar and caudal NP tissues yielded a mean RNA integrity number (RIN) of 9.6 and 9.8, respectively. Mouse lumbar and caudal AF tissues yielded a mean RIN of 8.9 and 8.3, respectively. Snap frozen NP and AF rat tissues yielded a mean RIN of 9.1 and 7.4, respectively. NP and AF tissues stored in Conclusions: This study describes methods that reproducibly generate high-quality RNA from freshly dissected and stored IVD tissues from mice and rats. Notably, we highlight a method using
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