ArticleScientific reports2026
Mitochondrial derived peptide humanin improved semen quality, semen freezability, antioxidant status and in-vitro fertility in crossbred bull.
Article in Scientific reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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1 citing paper in PubMed.
- Humanin ameliorates diabetes-induced testicular damage in a streptozotocin-induced mouse model.Experimental physiology · 2026Article
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Abstract
The sperm quality, freezability, and fertility of crossbred bulls exhibit significant unpredictability, which consequently impacts the breeding program. The present study documents the supplementation of humanin, a mitochondria-derived peptide, on crossbred bull's sperm quality, freezability, antioxidant status and in-vitro fertility. For this objective a total of 24 ejaculates, 8 from each of the three Vrindavani crossbred bulls, were selected based on sperm progressive motility (≥ 70%) and concentration (≥ 500 million). The selected fresh semen samples were divided into four aliquots and diluted with Tris-Fructose-Egg-Yolk-Glycerol (TFEGY) extender supplemented with various concentrations of humanin peptide; control (Group-1) without supplementation; Group II with 2.5 µM; Group III supplemented with 5 µM and Group IV supplemented with 7.5 µM. Semen quality parameters, sperm kinematics, sperm plasma membrane integrity, mitochondrial membrane potential, sperm cryo-capacitation status, ejaculate freezability rate, antioxidant status and in-vitro fertility assays were done. At the pre-freeze stage, there was no significant effect on the percentage of individual progressive motility in any group. Supplementation with 5 µM humanin resulted in an 87.5% ejaculate recovery rate after cryopreservation. At the post-thaw stage, sperm quality parameters, sperm kinematics, sperm plasma membrane integrity, mitochondrial membrane potential, sperm cryo-capacitation status, antioxidant status and in-vitro fertility were significantly improved in Group III (5 µM humanin supplementation). In conclusion, our results demonstrate that humanin supplementation in crossbred bull semen enhances semen freezability and post-thaw ejaculate recovery rate.
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