Evidence map›Paper›PMID 41632281›Full record

ArticleTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik2026

Gene editing and association analysis of circadian clock gene TaPRR59 highlights its importance in yield-related traits in wheat.

Jinrong Li, Meijie Zhu, Guimei Hu, Xiaoyue Chen, Huixian Xue, Yuanxun Zhang, Yifan Wang, Ziqi Li, Dengke Xu, Mingjun Zhai and 7 more

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Article in TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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5 · Who and what money

Authors and funding

17 authors.

Jinrong Li *College of Horticulture, Yantai Key Laboratory of Molecular Breeding for High-Yield and Stress-Resistant Crops and Efficient Cultivation, Ludong University, Yantai, Shandong, China.
Meijie Zhu *College of Horticulture, Yantai Key Laboratory of Molecular Breeding for High-Yield and Stress-Resistant Crops and Efficient Cultivation, Ludong University, Yantai, Shandong, China.
Guimei Hu *College of Horticulture, Yantai Key Laboratory of Molecular Breeding for High-Yield and Stress-Resistant Crops and Efficient Cultivation, Ludong University, Yantai, Shandong, China.
Xiaoyue ChenCollege of Horticulture, Yantai Key Laboratory of Molecular Breeding for High-Yield and Stress-Resistant Crops and Efficient Cultivation, Ludong University, Yantai, Shandong, China.
Huixian XueCollege of Horticulture, Yantai Key Laboratory of Molecular Breeding for High-Yield and Stress-Resistant Crops and Efficient Cultivation, Ludong University, Yantai, Shandong, China.
Yuanxun ZhangCollege of Horticulture, Yantai Key Laboratory of Molecular Breeding for High-Yield and Stress-Resistant Crops and Efficient Cultivation, Ludong University, Yantai, Shandong, China.
Yifan WangCollege of Horticulture, Yantai Key Laboratory of Molecular Breeding for High-Yield and Stress-Resistant Crops and Efficient Cultivation, Ludong University, Yantai, Shandong, China.
Ziqi LiCollege of Horticulture, Yantai Key Laboratory of Molecular Breeding for High-Yield and Stress-Resistant Crops and Efficient Cultivation, Ludong University, Yantai, Shandong, China.
Dengke XuCollege of Horticulture, Yantai Key Laboratory of Molecular Breeding for High-Yield and Stress-Resistant Crops and Efficient Cultivation, Ludong University, Yantai, Shandong, China.
Mingjun ZhaiCollege of Horticulture, Yantai Key Laboratory of Molecular Breeding for High-Yield and Stress-Resistant Crops and Efficient Cultivation, Ludong University, Yantai, Shandong, China.
Guangsheng ZhouCollege of Horticulture, Yantai Key Laboratory of Molecular Breeding for High-Yield and Stress-Resistant Crops and Efficient Cultivation, Ludong University, Yantai, Shandong, China.
Chenxiao CuiCollege of Horticulture, Yantai Key Laboratory of Molecular Breeding for High-Yield and Stress-Resistant Crops and Efficient Cultivation, Ludong University, Yantai, Shandong, China.
Chunhua ZhaoCollege of Horticulture, Yantai Key Laboratory of Molecular Breeding for High-Yield and Stress-Resistant Crops and Efficient Cultivation, Ludong University, Yantai, Shandong, China.
Ran QinCollege of Horticulture, Yantai Key Laboratory of Molecular Breeding for High-Yield and Stress-Resistant Crops and Efficient Cultivation, Ludong University, Yantai, Shandong, China.
Yongzhen WuCollege of Horticulture, Yantai Key Laboratory of Molecular Breeding for High-Yield and Stress-Resistant Crops and Efficient Cultivation, Ludong University, Yantai, Shandong, China. yongzhenwu1204@163.com.
Fa CuiCollege of Horticulture, Yantai Key Laboratory of Molecular Breeding for High-Yield and Stress-Resistant Crops and Efficient Cultivation, Ludong University, Yantai, Shandong, China. sdaucf@126.com.
Han SunCollege of Horticulture, Yantai Key Laboratory of Molecular Breeding for High-Yield and Stress-Resistant Crops and Efficient Cultivation, Ludong University, Yantai, Shandong, China. sunhan@ldu.edu.cn.ORCID http://orcid.org/0000-0003-3602-9860

Funding

Key R & D Program of Shandong Province, China (Major Innovation Project) 2022LZG002-2Major Basic Research Project of Natural Science Foundation of Shandong Province, China ZR2019ZD16National Natural Science Foundation for Young Scholars of China 32101726National Natural Science Foundation of China 32072051National Natural Science Foundation of China 32272119Natural Science Foundation of Shandong Province, China ZR2022MC119Shandong Provincial Fund for Excellent Young Scholars ZR2022YQ19Taishan scholar young expert 20230119The Innovation Project for graduate students of Ludong University IPGS2025-083Yantai science and technology plan project 2023ZDCX023
6 · The paper itself

Abstract

key messageMutations in TaPRR59 impact transcript levels of some key flowering genes and show earlier heading time and reduced plant height. Favorable haplotype TaPRR59-A1-Hapla was positively selected in wheat breeding programs. The circadian clock system is a crucial endogenous rhythmic regulatory mechanism with a significant role in plant growth and development. The pseudo-response regulator (PRR) family is a pivotal component of circadian networks. In the present study, we cloned the wheat PRR family member TaPRR59 and investigated its function using gene editing, transcriptome sequencing, haplotype analysis, and association analysis. The expression profile of TaPRR59 over a 24-h period exhibited a diurnal rhythmic expression pattern. Luciferase transient transcriptional assay demonstrated that TaPRR59 acts as a transcriptional repressor in the nucleus. The taprr59-ABD-KO gene-edited lines produced using the CRISPR/Cas9 genome-editing system had earlier heading time and reduced plant height. Overexpression of TaPRR59-D1 in rice significantly delayed the heading date, reduced plant height and thousand-grain weight, and increased the number of grains per panicle. Transcriptome analysis revealed the transcript levels of several key flowering genes and chlorophyll a-b binding protein-related genes were up- or down-regulated in the taprr59 mutant plants. Association analysis showed that natural variations at TaPRR59-A1, TaPRR59-B1, and TaPRR59-D1 were significantly associated with yield traits such as plant height, thousand-grain weight, and heading date. Geographical analysis showed distinctive distribution characteristics of TaPRR59 haplotypes in different agroecological production zones. Additionally, the significant difference in frequency of the favorable haplotype TaPRR59-A1-Hapla between landraces and modern cultivars indicates that it has been subject to directional selection during wheat breeding. This research provided novel insights into the influence of the circadian clock system on agronomic traits and provided useful molecular markers and genetic resources for wheat breeding.

Indexed as

Circadian ClocksGene EditingPlant ProteinsTriticumGene Expression Regulation, PlantGenes, PlantGenetic Association StudiesHaplotypesPhenotypePlant Proteins

Identifiers

PMID41632281

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.