Evidence map›Paper›PMID 41632257›Full record

ArticleACS nano2026

Quantitative Stimulated Emission Depletion (STED) Microscopy with DNA-Fluorophore Labels.

Laurell F Kessler, Yunqing Li, Ashwin Balakrishnan, Mike Heilemann

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in ACS nano, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Laurell F KesslerInstitute of Physical and Theoretical Chemistry, Goethe-University Frankfurt, Max-von-Laue-Strasse 7, Frankfurt 60438, Germany.
Yunqing LiInstitute of Physical and Theoretical Chemistry, Goethe-University Frankfurt, Max-von-Laue-Strasse 7, Frankfurt 60438, Germany.
Ashwin BalakrishnanInstitute of Physical and Theoretical Chemistry, Goethe-University Frankfurt, Max-von-Laue-Strasse 7, Frankfurt 60438, Germany.ORCID 0000-0003-4059-8345
Mike HeilemannInstitute of Physical and Theoretical Chemistry, Goethe-University Frankfurt, Max-von-Laue-Strasse 7, Frankfurt 60438, Germany.ORCID 0000-0002-9821-3578

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Stimulated emission depletion (STED) microscopy enables super-resolution imaging of complex biological samples in 3D, in large volumes, and live. However, molecular quantification with STED has remained underexplored. Here, we present a straightforward approach for quantitative STED that enables molecule counting. For this purpose, we designed DNA-fluorophore labels that enable signal amplification and allow for reliable intensity-based quantitative imaging. We demonstrate accurate molecule counting on DNA origami. Furthermore, we visualized and quantified EGF receptor monomers and dimers in cells. In summary, we introduce a robust, fast, and easy-to-implement tool for quantitative STED microscopy with single-protein resolution.

Indexed as

DNAFluorescent DyesDNA NanostructuresErbB ReceptorsHumansMicroscopy, FluorescenceDNAErbB ReceptorsFluorescent DyesEGFRmolecular countingquantitative microscopySTEDstimulated emission depletion

Identifiers

PMID41632257
PMCPMC12918720

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.