Transcriptomic Analysis from Cryosectioned Tissue Samples Using Laser Capture Microdissection-Based Geo-Seq.

Xianfa Yang, Yingying Chen, Yun Yang, Naihe Jing

Abstract read

In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

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2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

4 authors.

Xianfa YangGuangzhou National Laboratory, Guangzhou International Bio Island, Guangzhou, Guangdong, China. yang_xianfa@gzlab.ac.cn.ORCID https://orcid.org/0000-0002-6843-8055

Yingying ChenGuangzhou National Laboratory, Guangzhou International Bio Island, Guangzhou, Guangdong, China.ORCID https://orcid.org/0000-0002-0243-8730

Yun YangGuangzhou National Laboratory, Guangzhou International Bio Island, Guangzhou, Guangdong, China.ORCID https://orcid.org/0009-0005-8376-2151

Naihe JingGuangzhou National Laboratory, Guangzhou International Bio Island, Guangzhou, Guangdong, China. jing_naihe@gzlab.ac.cn.ORCID https://orcid.org/0000-0003-1509-6378

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Geographical position sequencing (Geo-seq), combining transcriptome sequencing and laser capture microdissection, enables comprehensive learning of gene expression within the spatial context. Geo-seq is robust for in-depth gene expression analyses from tissue section at specific position coordinate across various tissue types. The abundance of specific transcripts and the whole transcriptomic architecture can be determined following region-specific laser capture microdissection, RNA extraction, and cDNA preparation. Generally, Geo-seq enables high-throughput profiling of the transcriptomic architecture encompassing over 8000 genes from a single sampled spot.

Indexed as

CryoultramicrotomyGene Expression ProfilingLaser Capture MicrodissectionTranscriptomeAnimalsHigh-Throughput Nucleotide SequencingHumansRNASequence Analysis, RNARNALaser capture microdissectionReverse transcriptionRNA isolationTranscriptomic analysis

Identifiers

PMID41629711

DOI10.1038/s41467-023-41482-5

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.