Evidence map›Paper›PMID 41629422›Full record

ArticleScientific reports2026

Proteomic signatures of sperm and extracellular vesicles associated with sperm freezability in Holstein bulls.

Jinkang Cao, Bingwen Leng, Chun Zhang, Yachun Wang, Yi Zhang, Xianrui Zheng, Yuanpei Zhang, Yanqin Li, Li Jiang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Jinkang Cao *State Key Laboratory of Animal Biotech Breeding, National Engineering Laboratory For Animal Breeding, College of Animal Science & Technology, China Agricultural University, Beijing, 100193, China.
Bingwen Leng *State Key Laboratory of Animal Biotech Breeding, National Engineering Laboratory For Animal Breeding, College of Animal Science & Technology, China Agricultural University, Beijing, 100193, China.
Chun ZhangState Key Laboratory of Animal Biotech Breeding, National Engineering Laboratory For Animal Breeding, College of Animal Science & Technology, China Agricultural University, Beijing, 100193, China.
Yachun WangState Key Laboratory of Animal Biotech Breeding, National Engineering Laboratory For Animal Breeding, College of Animal Science & Technology, China Agricultural University, Beijing, 100193, China.
Yi ZhangState Key Laboratory of Animal Biotech Breeding, National Engineering Laboratory For Animal Breeding, College of Animal Science & Technology, China Agricultural University, Beijing, 100193, China.
Xianrui ZhengState Key Laboratory of Animal Biotech Breeding, National Engineering Laboratory For Animal Breeding, College of Animal Science & Technology, China Agricultural University, Beijing, 100193, China.
Yuanpei ZhangShandong OX Livestock Breeding Co., Ltd, Jinan, 250100, China.
Yanqin LiInstitute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Jinan, 250100, China.
Li JiangState Key Laboratory of Animal Biotech Breeding, National Engineering Laboratory For Animal Breeding, College of Animal Science & Technology, China Agricultural University, Beijing, 100193, China. lijiang@cau.edu.cn.

Funding

National Key Research and Development Program of China 2022YFD1302200National Natural Science Foundation 3210200137the earmarked fund for CARS CARS36
6 · The paper itself

Abstract

Semen cryopreservation and artificial insemination have crucial and beneficial effects on cattle breeding. The freezability of sperm, as primarily reflected by post-thaw sperm motility (PTM), is essential for evaluating semen quality. Some studies have shown notable differences in sperm freezability among various bulls. Here, we compared protein profiles of sperm cells and seminal plasma extracellular vesicles (SPEVs) in the high sperm freezability group and the low sperm freezability group of Holstein bulls to identify the important proteins and their mechanisms that affect sperm freezability. As a result, 432 and 394 differentially expressed proteins (DEPs) were identified in sperm and SPEVs between high and low freezability groups. The results of weighted correlation network analysis (WGCNA) showed that the blue module was significantly (r = 0.89, P = 9 × 10

Indexed as

CryopreservationExtracellular VesiclesProteomeProteomicsSemen PreservationSpermatozoaAnimalsCattleMaleSemen AnalysisSperm MotilitySperm ProteinsProteomeSperm ProteinsExtracellular vesiclesFreezabilityHSPA1AProteomeSperm

Identifiers

PMID41629422
PMCPMC12916939

What OpenQuestion holds

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LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.