Evidence map›Paper›PMID 41627765›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2026

Isolation of Mitochondria and Chloroplasts from Euglena gracilis Z.

Shun Tamaki, Takahiro Ishikawa

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Shun TamakiDepartment of Biological Science, Faculty of Science and Engineering, Yasuda Women's University, Asaminami-ku, Hiroshima, Japan.
Takahiro IshikawaDepartment of Life Sciences, Faculty of Life and Environmental Science, Shimane University, Matsue, Shimane, Japan. ishikawa@life.shimane-u.ac.jp.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Euglena gracilis has emerged as the most extensively investigated model organism within the euglenid protists, yielding a substantial body of biological insights to date. This species has undergone a distinctive evolutionary trajectory via secondary endosymbiosis, and its metabolic profile exhibits both heterotrophic and photoautotrophic features, contingent upon culture conditions and environmental stimuli. The isolation of mitochondria and chloroplasts not only serves as a means for conducting detailed physiological and biochemical analyses of these organelles but also facilitates targeted proteomic and metabolomic investigations. These approaches are expected to significantly advance the understanding of the biological properties of E. gracilis. This chapter presents the methodology employed in our laboratory for the isolation of mitochondria and chloroplasts from E. gracilis.

Indexed as

Cell FractionationChloroplastsEuglena gracilisMitochondriaCell disruptionChloroplastsEuglena gracilisMitochondriaOrganelle isolation

Identifiers

PMID41627765

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.