Evidence map›Paper›PMID 41627764›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2026

Euglena gracilis Subcellular Fractionation.

Lucia Tomečková, Vladimír Hampl, Priscila Peña-Diaz

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Lucia TomečkováDepartment of Parasitology, Faculty of Science, Charles University, BIOCEV, Vestec, Czech Republic.
Vladimír HamplDepartment of Parasitology, Faculty of Science, Charles University, BIOCEV, Vestec, Czech Republic.
Priscila Peña-DiazDepartment of Parasitology, Faculty of Science, Charles University, BIOCEV, Vestec, Czech Republic. penadiac@natur.cuni.cz.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Subcellular fractionation of Euglena gracilis has been conducted for over 50 years in various forms by numerous research groups. The development of this technique is closely tied to the specific organelle or fraction required for specific purposes. In this chapter, we describe our approach to this process and discuss the insights we gain from it. Sucrose and iodixanol gradients are employed to separate the main organelles of interest; however, these methods alone do not lead to the complete purification of the organelles.

Indexed as

Cell FractionationEuglena gracilisSubcellular FractionsCentrifugation, Density GradientCentrifugation, IsopycnicOrganellesSucroseTriiodobenzoic AcidsiodixanolSucroseTriiodobenzoic AcidsEuglenaGradientsIsopycnic centrifugationOrganelle isolationSubcellular distributionSubcellular fractionation

Identifiers

PMID41627764

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.