ArticleMethods in molecular biology (Clifton, N.J.)2026
Tetracycline-Inducible Functional Complementation Analysis in Trypanosoma Parasites.
Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Euglenozoa is a diverse group of protists that includes euglenids, kinetoplastids, diplonemids, and symbiontids, with phototrophic euglenids and parasitic trypanosomatids being the most extensively studied. Among these, Trypanosoma brucei is a key model organism for molecular and cellular parasitology, supported by well-established genetic tools. Among these, RNA interference (RNAi) is widely used for targeted gene knockdown and functional genomics. RNAi knockdown, combined with functional complementation using RNAi-resistant constructs provides a powerful means to confirm the specificity of RNAi-induced phenotypes and to assess the function of genes. This chapter outlines a detailed protocol for the functional complementation of RNAi growth phenotype using codon-exchanged, RNAi-resistant constructs. Using the essential glycosomal membrane protein PEX15 as an example, we demonstrate that both full-length and mutant variants can be tested for their function and the dissection of essential regions in a protein. This combined approach confirms gene essentiality, reveals the roles of specific protein domains, and provides a robust framework for studying protein function and interactions in trypanosomatids and other euglenozoans with a functional RNAi pathway.
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