Evidence map›Paper›PMID 41627754›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2026

Application of Double-Inducible Expression System in Bloodstream-Form Trypanosoma brucei.

Lisa Hohnen, Ralf Erdmann, Vishal C Kalel

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Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Lisa HohnenDepartment of Systems Biochemistry, Institute for Biochemistry and Pathobiochemistry, Faculty of Medicine, Ruhr University Bochum, Bochum, Germany.
Ralf ErdmannDepartment of Systems Biochemistry, Institute for Biochemistry and Pathobiochemistry, Faculty of Medicine, Ruhr University Bochum, Bochum, Germany. ralf.erdmann@ruhr-uni-bochum.de.
Vishal C KalelDepartment of Systems Biochemistry, Institute for Biochemistry and Pathobiochemistry, Faculty of Medicine, Ruhr University Bochum, Bochum, Germany. vishal.kalel@ruhr-uni-bochum.de.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Gene functions in Trypanosoma brucei parasites are investigated by several reverse genetic approaches. One of the widely used tools is the tetracycline-inducible system, which allows controlled overexpression of a gene of interest or knockdown of gene expression by RNA interference. For studying multiple genes, it is desirable to be able to control their expression independently or together, as required. A tetracycline- and vanillic acid-based double-inducible expression system was previously described for procyclic-form trypanosomes. Here, we describe the adaptation of this double inducible system to bloodstream-form trypanosomes. We show that this system can be used for stringent, independent, and simultaneous regulation of the expression of two genes.

Indexed as

Gene Expression RegulationTrypanosoma brucei bruceiGene ExpressionRNA InterferenceTetracyclineVanillic AcidTetracyclineVanillic AcidBloodstream formDouble-inducible systemGene expressionTetracyclineTrypanosoma bruceiVanillic acid

Identifiers

PMID41627754

What OpenQuestion holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.