ArticleMethods in molecular biology (Clifton, N.J.)2026
Multi-Approach Workflows for Chromatin and Histone Isolation from Trypanosoma cruzi: From Basic Protein Extraction to Mass Spectrometry-Compatible Protocols.
Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
This chapter presents a comprehensive set of protocols for isolating chromatin and histones from Trypanosoma cruzi, tailored for applications ranging from gel-based analyses to high-resolution mass spectrometry. It outlines optimized workflows for extracting basic nuclear proteins, histones, linker histone H1, and chromatin-associated proteins, adapted to different parasite life stages and cell cycle phases. By integrating multiple approaches, these methods address challenges posed by the parasite's unique biology and provide flexible tools for studying histone post-translational modifications and chromatin proteins. The inclusion of protocols compatible with proteomic workflows supports broader investigations into epigenetic regulation and nuclear processes in T. cruzi.
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