ArticleMethods in molecular biology (Clifton, N.J.)2026
Genetic Manipulation of Paradiplonema papillatum.
Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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1 citing paper in PubMed.
- Identification of feeding apparatus components in a heterotrophic marine flagellate.Journal of cell science · 2026Article
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6 authors.
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Abstract
Here we present a collection of methods established for genetic manipulation of Paradiplonema papillatum (also referred to as Diplonema papillatum), so far the only diplonemid species that is genetically tractable and can therefore serve as a model organism, enabling functional studies of this very abundant and species-rich group of marine protists. We describe a complete transformation protocol which includes: (1) the Alamar blue assay, a method that serves to identify antibiotics, to which P. papillatum is sensitive and can be used for transformant selection; (2) the design and preparation of DNA constructs for functional genomics; and (3) two protocols for electroporation and transformant screening. We conclude with an immunoprecipitation protocol optimized for pulling down target-tagged proteins along with their interaction partners, allowing the characterization of protein complexes.
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