Evidence map›Paper›PMID 41627739›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2026

Small RNA Profiling in Trypanosomes.

Florencia Díaz-Viraqué, Gonzalo Greif, María Rosa García-Silva, Carlos Robello

Abstract read
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Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Florencia Díaz-ViraquéLaboratorio de Interacciones Hospedero-Patógeno-UBM, Institut Pasteur de Montevideo, Montevideo, Uruguay.
Gonzalo GreifLaboratorio de Interacciones Hospedero-Patógeno-UBM, Institut Pasteur de Montevideo, Montevideo, Uruguay.
María Rosa García-SilvaLaboratorio de Interacciones Hospedero-Patógeno-UBM, Institut Pasteur de Montevideo, Montevideo, Uruguay.
Carlos RobelloLaboratorio de Interacciones Hospedero-Patógeno-UBM, Institut Pasteur de Montevideo, Montevideo, Uruguay. robello@pasteur.edu.uy.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Trypanosomatids express a diverse repertoire of small non-coding RNAs despite lacking the canonical RNA interference machinery in most species. These small RNAs-including fragments derived from tRNAs, rRNAs, and other non-coding sources-are thought to play regulatory roles in parasite biology and host-parasite interactions. In this chapter, we provide a detailed protocol for the isolation, library preparation, sequencing, and computational analysis of small RNAs in trypanosomes. The method is optimized to preserve short RNA species while minimizing artifacts such as adaptor dimers and concatamerization. It includes quality control and size selection steps to ensure the reproducibility and high quality of the libraries, allowing the study of the transcriptome of small RNAs in these parasites.

Indexed as

Gene Expression ProfilingRNA, ProtozoanRNA, Small UntranslatedTrypanosomaGene LibraryHigh-Throughput Nucleotide SequencingSequence Analysis, RNATranscriptomeRNA, ProtozoanRNA, Small UntranslatedNext-generation sequencingNon-coding RNAsProtist parasitesSmall RNAsTrypanosomes

Identifiers

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.