Evidence map›Paper›PMID 41627732›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2026

Poly(A) Profiling for Euglenozoans Using Oxford Nanopore Direct RNA Sequencing, Illustrated by the Example of Euglena gracilis.

Natalia Gumińska, Agnieszka Czarnocka-Cieciura, Bożena Zakryś, Rafał Milanowski

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Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

4 authors.

Natalia GumińskaInternational Institute of Molecular and Cell Biology, Laboratory of RNA Biology, Warsaw, Poland. nguminska@iimcb.gov.pl.
Agnieszka Czarnocka-CieciuraInternational Institute of Molecular and Cell Biology, Laboratory of RNA Biology, Warsaw, Poland.
Bożena ZakryśFaculty of Biology, Institute of Evolutionary Biology, University of Warsaw, Warsaw, Poland.
Rafał MilanowskiFaculty of Biology, Institute of Evolutionary Biology, University of Warsaw, Warsaw, Poland.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

There are numerous methods to study polyadenylation. These techniques offer insights into mRNA abundance and the dynamic regulation of poly(A) tail length and nucleotide content. The Oxford Nanopore Technologies (ONT) platform is particularly noteworthy among them. It allows analysis of the whole transcriptome at the single-molecule level without the amplification biases introduced by PCR-based methods.Most current studies in this area have focused on well-established model organisms such as humans, yeast, Arabidopsis, and Caenorhabditis elegans. In contrast, the poly(A) dynamics remains largely unexplored in non-model organisms like euglenids. These organisms possess a range of unique evolutionary traits, and investigating their poly(A) metabolism may elucidate the evolution of the enzymatic machinery that controls mRNA processing, stability, and turnover.Here, we present a step-by-step protocol for measuring poly(A) tail lengths and characterizing the nucleotide makeup of poly(A) tails in Euglena gracilis using ONT Direct RNA Sequencing.

Indexed as

Euglena gracilisNanopore SequencingPoly ARNA, MessengerSequence Analysis, RNAAnimalsGene Expression ProfilingPolyadenylationTranscriptomePoly ARNA, MessengerDirect RNA sequencingEuglena gracilisEuglenidsNanoporeNon-adenosinePoly(A)

Identifiers

PMID41627732

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.