Evidence map›Paper›PMID 41627731›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2026

Culture and Genetic Modification of the Monoxenous Trypanosomatids Crithidia fasciculata and Crithidia bombi.

Blyssalyn V Bieber, Faith A St Clair, Jesse Quatse, Shane Denecke, Michael Povelones, Megan L Povelones

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. The trypanosomatid dynamin-like protein associates with glycosomes.bioRxiv : the preprint server for biology · 2026
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Blyssalyn V BieberDepartment of Biology, Villanova University, Villanova, PA, USA.
Faith A St ClairDepartment of Biology, Villanova University, Villanova, PA, USA.
Jesse QuatseDepartment of Pathobiology, University of Pennsylvania School of Veterinary Medicine, Philadelphia, PA, USA.
Shane DeneckeDepartment of Pathobiology, University of Pennsylvania School of Veterinary Medicine, Philadelphia, PA, USA.
Michael PovelonesDepartment of Biology, Villanova University, Villanova, PA, USA.
Megan L PovelonesDepartment of Biology, Villanova University, Villanova, PA, USA. megan.povelones@villanova.edu.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Monoxenous trypanosomatids exclusively parasitize insects and are powerful systems for exploring trypanosomatid biology, the evolution of parasitism, and host-parasite interactions. In addition, they are tractable early-diverging eukaryotes that can serve as model organisms for a variety of conserved processes. We have elaborated on existing methods for culturing the mosquito parasite Crithidia fasciculata and the bumblebee parasite Crithidia bombi, including quantitative assessment of surface attachment and genetic modification. In addition, as cultured strains retain their ability to infect their insect hosts, we describe use of a luciferase-based reporter line to quantitate infection intensities in laboratory mosquitoes.

Indexed as

CrithidiaCrithidia fasciculataAnimalsCulicidaeHost-Parasite InteractionsLuciferasesLuciferasesAdhesionAttachmentCrithidiaLuciferaseNucleofection

Identifiers

PMID41627731

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.