ArticleMethods in molecular biology (Clifton, N.J.)2026
Preparation of Diplonemid Samples for Microscopy.
Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Authors and funding
6 authors.
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Abstract
This chapter provides a comprehensive set of protocols for preparing diplonemid cells for a range of microscopy applications, including live-cell imaging, fixed-cell fluorescence microscopy, endocytosis assay, immunofluorescence assay (IFA), and fluorescence in situ hybridization (FISH). It presents detailed instructions for slide preparation, cell harvesting, chemical fixation, permeabilization, and fluorescent labelling, with critical steps highlighted throughout. These protocols are designed to be adaptable for different experimental goals, enabling researchers to obtain high-quality imaging data for exploring the cellular biology of diplonemids.
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Registered trials
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