Evidence map›Paper›PMID 41627728›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2026

Isolation and Cultivation of Diplonemids.

Daria Tashyreva, Galina Prokopchuk, Orsola Iorillo, Matus Valach, Ingrid Škodová-Sveráková, Dong-Woo Shin, James A Letts, Gertraud Burger, Julius Lukeš, Drahomíra Faktorová

Abstract read
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Daria TashyrevaInstitute of Evolutionary Biology, Faculty of Biology, University of Warsaw, Warsaw, Poland.ORCID https://orcid.org/0000-0002-1280-4095
Galina ProkopchukInstitute of Parasitology, Biology Centre, Czech Academy of Sciences, České Budějovice (Budweis), Czech Republic.ORCID https://orcid.org/0000-0002-7623-5354
Orsola IorilloInstitute of Parasitology, Biology Centre, Czech Academy of Sciences, České Budějovice (Budweis), Czech Republic.ORCID https://orcid.org/0009-0008-2416-5957
Matus ValachDepartment of Biochemistry and Robert-Cedergren Centre for Bioinformatics and Genomics, Université de Montréal, Montréal, QC, Canada.ORCID https://orcid.org/0000-0001-8689-0080
Ingrid Škodová-SverákováInstitute of Parasitology, Biology Centre, Czech Academy of Sciences, České Budějovice (Budweis), Czech Republic.ORCID https://orcid.org/0000-0002-4004-3377
Dong-Woo ShinDepartment of Molecular and Cellular Biology, University of California, Davis, CA, USA.ORCID https://orcid.org/0000-0003-0373-5590
James A LettsDepartment of Molecular and Cellular Biology, University of California, Davis, CA, USA.ORCID https://orcid.org/0000-0002-9864-3586
Gertraud BurgerDepartment of Biochemistry and Robert-Cedergren Centre for Bioinformatics and Genomics, Université de Montréal, Montréal, QC, Canada.ORCID https://orcid.org/0000-0002-8679-8812
Julius LukešInstitute of Parasitology, Biology Centre, Czech Academy of Sciences, České Budějovice (Budweis), Czech Republic.ORCID https://orcid.org/0000-0002-0578-6618
Drahomíra FaktorováInstitute of Parasitology, Biology Centre, Czech Academy of Sciences, České Budějovice (Budweis), Czech Republic. dranov@paru.cas.cz.ORCID https://orcid.org/0000-0001-9623-2233

Funding

Understanding the Mechanisms of Respiratory Supercomplexes and mitochondrial Complex IR35GM137929 · NIGMS · UNIVERSITY OF CALIFORNIA AT DAVIS · PI LETTS, JAMES ANTHONY · 2020 to 2025
$2.3M
NIGMS NIH HHS R35 GM137929
6 · The paper itself

Abstract

Marine diplonemids (Diplonemidae and Hemistasiidae), once considered difficult to culture, can now be introduced into stable axenic cultures and grown to high densities. This chapter provides detailed protocols on establishing clonal cultures from environmental cells, their axenization and routine maintenance, as well as instructions on mid- and large-scale cultivation, estimation of cell density, and cryopreservation of selected species.

Indexed as

Axenic CultureCell Culture TechniquesAnimalsCryopreservationAxenizationCryopreservationCultivationDiplonemidsIsolationLarge-scale cultivation

Identifiers

PMID41627728
PMCPMC13641885

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.