Evidence map›Paper›PMID 41627584›Full record

ArticleMolecular biology reports2026

Epigenetic and MicroRNA signatures as predictive biomarkers in HCV genotype 4-Induced liver cirrhosis and HCC.

Rady E El-Araby, Mahmoud A Khalifa, Mona M Zoheiry, Manal Y Zahran, Faiza M Essawy, Mohamed I Rady, Raafat A Ibrahim, Mohamed D El-Talkawy, Alaa Gad

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Article in Molecular biology reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

9 authors.

Rady E El-ArabyCentral Lab, Theodor Bilharz Research Institute (TBRI), Ministry of Scientific Research, Giza, Egypt. radyeid.elaraby@gmail.com.ORCID http://orcid.org/0000-0003-4822-5279
Mahmoud A KhalifaBioinformatics and Molecular Biology Unit, Zoology Department, Faculty of Science, Al-Azhar University, Cairo, Egypt.ORCID http://orcid.org/0000-0001-7704-1722
Mona M ZoheiryImmunology Research Department, Theodor Bilharz Research Institute (TBRI) Ministry of Scientific Research, Giza, Egypt.
Manal Y ZahranHematology lab, Clinical Laboratory Research Department, Theodor Bilharz Research Institute (TBRI), Ministry of Scientific Research, Giza, Egypt.
Faiza M EssawyHematology lab, Clinical Laboratory Research Department, Theodor Bilharz Research Institute (TBRI), Ministry of Scientific Research, Giza, Egypt.
Mohamed I RadyCytochemistry and Histochemistry Lab, Zoology Department Faculty of Science, Al-Azhar University, Cairo, Egypt.
Raafat A IbrahimHepatoastroenterology Department, Theodor Bilharz Research Institute (TBRI), Ministry of Scientific Research, Gizah, Egypt.
Mohamed D El-TalkawyHepatoastroenterology Department, Theodor Bilharz Research Institute (TBRI), Ministry of Scientific Research, Gizah, Egypt.
Alaa GadClinical and Chemical Pathology Department, Faculty of Medicine, Cairo University, 6 A Aswan Square, El Mohandeseen, Cairo, Egypt. Alaa.gad@kasralainy.edu.eg.

Funding

Theodor Bilharz Research Institute R24
6 · The paper itself

Abstract

introductionHepatocellular carcinoma (HCC) and chronic liver disease (CLD) are mostly linked to hepatitis C virus (HCV) infection, especially genotype 4. The advancement of liver cancer is significantly influenced by epigenetic mechanisms, such as DNA methylation and microRNA regulation. AIM OF THE STUDY: This study aimed to assess the expression of two tumor suppressor genes in patients infected with HCV genotype 4: glutathione S-transferase pi 1 (GSTP1) and E-cadherin 1 (CDH1). It also sought to investigate the regulatory interactions among these genes, microRNA152 (miRNA152), and DNA methyltransferase 1 (DNMT1) to assess their potential as biomarkers for hepatocellular carcinoma (HCC) development.

methodsQuantitative real-time PCR was done to evaluate the gene expression levels of GSTP1, CDH1, miRNA152, and DNMT1 in CLD and HCC patients infected with HCV genotype 4. Correlation analyses were used to examine regulatory linkage.

resultsThe results indicated a substantial inverse correlation between DNMT1 and miRNA152. Furthermore, DNMT1 expression exhibited a negative association with both GSTP1 and CDH1, while GSTP1 and CDH1 had a positive correlation. The results indicate that DNMT1 and miRNA152 govern the epigenetic inactivation of tumor suppressor genes.

conclusionIn individuals with HCV genotype 4, the interplay of miRNA152, DNMT1, GSTP1, and CDH1 may contribute to the pathogenesis of HCC. These indicators demonstrate potential roles as therapeutic targets and noninvasive prognostic biomarkers for HCV-related liver disease.

Indexed as

Carcinoma, HepatocellularHepacivirusLiver CirrhosisLiver NeoplasmsMicroRNAsAdultAntigens, CDBiomarkers, TumorCadherinsDNA (Cytosine-5-)-Methyltransferase 1DNA MethylationEpigenesis, GeneticFemaleGene Expression Regulation, NeoplasticGenotypeGlutathione S-Transferase piAntigens, CDBiomarkers, TumorCadherinsCDH1 protein, humanDNA (Cytosine-5-)-Methyltransferase 1DNMT1 protein, humanGlutathione S-Transferase piGSTP1 protein, humanMicroRNAsCDH1CirrhosisCLDDNMT1Genotype 4GSTP1HCCHCVMiRNA152

Identifiers

PMID41627584

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