ArticleMycopathologia2026
In Silico Analysis and Expression Profiling of Adhesin-Like Flocculins in Trichosporon asahii.
Article in Mycopathologia, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
backgroundTrichosporon asahii is an opportunistic basidiomycetous yeast increasingly recognized as a cause of invasive infections in immunocompromised individuals, often associated with high morbidity and mortality. Its ability to adhere to host tissues and abiotic surfaces is considered a critical virulence factor, yet the molecular mechanisms underlying this adhesion remain poorly understood.
objectiveTo characterize gene sequences in T. asahii belonging to the flocculin family with potential adhesive functions.
methodsThe homologous flocculin genes DHA1, DHA2, CPL1, and CFL1, previously described in Cryptococcus neoformans as being involved in cell adhesion, were identified within the T. asahii predicted proteome. Four T. asahii strains were analyzed: two reference strains (CBS2479 and CBS7631) and two clinical strains (L2585 and L773). Cells were grown in planktonic morphology and biofilms in RPMI + MOPS medium with 2% glucose. The predominant morphology of the cells was observed using bright-field optical microscopy, and total RNA was extracted. The relative expression of the four flocculin genes was analyzed by qRT-PCR and normalized using the 2-ΔΔCt method and the actin gene.
resultsThe flocculin proteins showed high similarity and common domains with C. neoformans flocculins and other adhesins. The strains exhibited a variety of morphologies, with the T. asahii CBS2479 strain predominantly displaying yeast forms, while the T. asahii L2585 strain presented a higher quantity of hyphae. All flocculins bound to human proteins in the PriaA/Cpl1_fungi domain and/or to the Cpl1-like domain. T. asahii flocculins were downregulated when compared to the reference strain CBS2479, while the clinical strain L2585 exhibited the highest expression of the flocculin CFL1. During biofilm formation, DHA1 and CFL1 were highly expressed.
conclusionFlocculins were expressed according to specific yeast/hyphal morphology and were also more expressed in biofilms, indicating their association with biofilm maintenance and development.
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