Proteolytic extracellular enzymes of Pseudomonas fragi drive spoilage metabolism in grouper (Epinephelus lanceolatus) during cold storage: insights into degradation pathways.

Zixin Chen, Bingyu Chen, Jing Xie, Jun Mei

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Article in Archives of microbiology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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4 authors.

Zixin ChenCollege of Food Science and Technology, Shanghai Ocean University, Shanghai, 201306, China.

Bingyu ChenCollege of Food Science and Technology, Shanghai Ocean University, Shanghai, 201306, China.

Jing XieCollege of Food Science and Technology, Shanghai Ocean University, Shanghai, 201306, China. jxie@shou.edu.cn.

Jun MeiCollege of Food Science and Technology, Shanghai Ocean University, Shanghai, 201306, China. jmei@shou.edu.cn.

Funding

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6 · The paper itself

Abstract

The research investigated the biochemical properties of spoilage bacteria Pseudomonas fragi (P. fragi) in grouper (Epinephelus lanceolatus) during cold storage. The proteolytic activity and the spoilage-related metabolic pathway were characterized by whole genome sequencing. The results revealed optimal protease activity at pH 7.0 and 40 °C, with significant inhibition observed in the presence of protease inhibitors, organic solvents, and metal ions. The kinetic analysis demonstrated strong substrate affinity, while the thermodynamic parameters indicated excellent thermal stability. Freshness index evaluations confirmed the potential of fish protein degradation of P. fragi. The whole genome sequencing has made gene-level protease analysis a crucial approach for investigating protease metabolism. Genes associated with protein degradation and spoilage pathways were identified. The results revealed that both P. fragi V11 and SS strains contain 36 protease-encoding genes, with metalloprotease and serine protease representing the predominant proteases in P. fragi. These identified genes functioned in regulating amine, sulfur and amino acid metabolism. Whole genome sequencing may offer valuable insights into protease mechanisms and protein degradation processes.

Indexed as

Bacterial ProteinsBassPeptide HydrolasesPseudomonas fragiSeafoodAnimalsCold TemperatureFood StorageGenome, BacterialHydrogen-Ion ConcentrationMetabolic Networks and PathwaysProteolysisWhole Genome SequencingBacterial ProteinsPeptide HydrolasesGrouperProteolytic activitySpoilage bacteriaWhole genome sequencing

Identifiers

PMID41627472

DOI10.1016/j.fbio.2024.104480

What OpenQuestion holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.