Evidence map›Paper›PMID 41627457›Full record

ArticleArchives of microbiology2026

Isolation and molecular characterization of gut-derived lactic acid bacteria from the gangetic mystus, Mystus Cavasius with promising probiotic attributes.

Chandrika Mondal, Md Sakhawat Hossain, Rafiatul Jannat Rifa, Md Imtiaz Ahamed, Tanjila Akhter Tanni, Zakir Hossain, Tanvir Rahman, Mahbubul Pratik Siddique, Mariom

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Article in Archives of microbiology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

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4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Chandrika MondalDepartment of Fisheries Biology and Genetics, Faculty of Fisheries, Bangladesh Agricultural University, Mymensingh, 2202, Bangladesh.
Md Sakhawat HossainDepartment of Fisheries Biology and Genetics, Faculty of Fisheries, Bangladesh Agricultural University, Mymensingh, 2202, Bangladesh.
Rafiatul Jannat RifaDepartment of Fisheries Biology and Genetics, Faculty of Fisheries, Bangladesh Agricultural University, Mymensingh, 2202, Bangladesh.
Md Imtiaz AhamedDepartment of Fisheries Biology and Genetics, Faculty of Fisheries, Bangladesh Agricultural University, Mymensingh, 2202, Bangladesh.
Tanjila Akhter TanniDepartment of Fisheries Biology and Genetics, Faculty of Fisheries, Bangladesh Agricultural University, Mymensingh, 2202, Bangladesh.
Zakir HossainDepartment of Fisheries Biology and Genetics, Faculty of Fisheries, Bangladesh Agricultural University, Mymensingh, 2202, Bangladesh.
Tanvir RahmanDepartment of Aquaculture, Faculty of Fisheries, Bangladesh Agricultural University, Mymensingh, 2202, Bangladesh.
Mahbubul Pratik SiddiqueDepartment of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh, 2202, Bangladesh.
MariomDepartment of Fisheries Biology and Genetics, Faculty of Fisheries, Bangladesh Agricultural University, Mymensingh, 2202, Bangladesh. mariom.fbg@bau.edu.bd.ORCID https://orcid.org/0000-0002-3075-8117

Funding

Bangladesh Agricultural University Research System BAURES/2023/49/BAU
6 · The paper itself

Abstract

Probiotics are live microorganisms that confer health benefits when administered in adequate amounts, enhance immune function, promote gut health, and are increasingly recognized as effective alternatives to antibiotics for improving animal health and productivity. This study investigated the probiotic potential of lactic acid bacteria (LAB) from Mystus cavasius through morphological, physiological, and molecular characterization for aquaculture feed applications. Out of twenty-two presumptive LAB isolates, eleven were sequenced after biochemical screening, and molecular analysis confirmed eight as LAB (Weisella confusa ML1, ML2, ML3, ML6, ML7; Enterococcus faecalis ML8, ML9; Lactococcus garvieae ML10). The study also found a potential non-LAB probiotic bacterium Bacillus subtilis ML4. Moreover, the study identified Paraburkholderia phytofirmans, a potential plant probiotic bacterium, for the first time in fish. Among the identified LAB isolates, L. garvieae ML10 was excluded from probiotic potential assessments because of its reported disease-causing potential and associated health risks. All the tested LAB isolates were found to tolerate acidic pH (2–5) and bile salts (2.5–7.5%), indicating their probiotic potential for aquaculture applications. Additionally, the isolates showed no hemolytic activity and demonstrated strong probiotic potential, characterized by high cell surface hydrophobicity, ranging from 41.98 ± 16.68% to 62.19 ± 4.09% in xylene and 51.58 ± 1.67% to 64.83 ± 4.91% in toluene, robust autoaggregation (55.51 ± 3.63% to 64.33 ± 2.87%) and substantial coaggregation with Staphylococcus haemolyticus (46.27 ± 2.96% to 53.54 ± 0.72%) and Bacillus cereus (34.34 ± 3.16% to 51.76 ± 4.08%). Furthermore, they exhibited potent in vitro inhibitory effects against the fish pathogens Aeromonas veronii and L. garvieae and revealed susceptibility to several commonly used antibiotics. Therefore, the results revealed that the selected LAB isolates might be ideal probiotic candidates to be used in sustainable aquaculture practices.

Indexed as

Gastrointestinal TractLactobacillalesProbioticsAnimalsAnti-Bacterial AgentsAquacultureHydrogen-Ion ConcentrationPhylogenyRNA, Ribosomal, 16SAnti-Bacterial AgentsRNA, Ribosomal, 16S16S rRNA geneAntibiotic susceptibilityLactic acid bacteriaMystus cavasiusProbiotic potential

Identifiers

PMID41627457

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.