Evidence map›Paper›PMID 41626694›Full record

ReviewNucleic acids research2026

Unlocking the full potential of nanopore sequencing: tips, tricks, and advanced data analysis techniques.

Daria Meyer, Winfried Goettsch, Jannes Spangenberg, Bettina Stieber, Sebastian Krautwurst, Martin Hölzer, Christian Brandt, Jörg Linde, Christian Höner Zu Siederdissen, Akash Srivastava and 3 more

Abstract readReview
In one paragraph

Review in Nucleic acids research, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Review
  5. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Daria MeyerRNA Bioinformatics and High-Throughput Analysis, Friedrich Schiller University, Leutragraben 1, 07743 Jena, Germany.ORCID 0000-0003-2006-4610
Winfried GoettschRNA Bioinformatics and High-Throughput Analysis, Friedrich Schiller University, Leutragraben 1, 07743 Jena, Germany.
Jannes SpangenbergRNA Bioinformatics and High-Throughput Analysis, Friedrich Schiller University, Leutragraben 1, 07743 Jena, Germany.ORCID 0000-0002-6126-411X
Bettina StieberOncgnostics GmbH, Löbstedter Str. 41, 07749 Jena, Germany.
Sebastian KrautwurstRNA Bioinformatics and High-Throughput Analysis, Friedrich Schiller University, Leutragraben 1, 07743 Jena, Germany.ORCID 0000-0002-9413-5701
Martin HölzerEuropean Virus Bioinformatics Center, Friedrich Schiller University, Leutragraben 1, 07743 Jena, Germany.ORCID 0000-0001-7090-8717
Christian BrandtInstitute of Infectious Diseases and Infection Control, Jena University Hospital, Friedrich Schiller University, 07747 Jena, Germany.
Jörg LindeEuropean Virus Bioinformatics Center, Friedrich Schiller University, Leutragraben 1, 07743 Jena, Germany.
Christian Höner Zu SiederdissenRNA Bioinformatics and High-Throughput Analysis, Friedrich Schiller University, Leutragraben 1, 07743 Jena, Germany.ORCID 0000-0001-9517-5839
Akash SrivastavaRNA Bioinformatics and High-Throughput Analysis, Friedrich Schiller University, Leutragraben 1, 07743 Jena, Germany.
Milena ZarkovicRNA Bioinformatics and High-Throughput Analysis, Friedrich Schiller University, Leutragraben 1, 07743 Jena, Germany.
Damian WollnyRNA Bioinformatics and High-Throughput Analysis, Friedrich Schiller University, Leutragraben 1, 07743 Jena, Germany.
Manja MarzRNA Bioinformatics and High-Throughput Analysis, Friedrich Schiller University, Leutragraben 1, 07743 Jena, Germany.

Funding

Deutsche Forschungsgemeinschaft 390713860Friedrich-Schiller University JenaGerman DFG Collaborative Research Centre AquaDiva CRC 1076 AquaDivaGerman state of Thuringia via the Thüringer Aufbaubank 2021 FGI 0009Landesprogramm ProDigital DigLeben-5575/10-9Ministry for Economics, Sciences, and Digital Society of Thuringia
6 · The paper itself

Abstract

Nucleic acid sequencing is the process of identifying the sequence of DNA or RNA, with DNA used for genomes and RNA for transcriptomes. Deciphering this information has the potential to greatly advance our understanding of genomic features and cellular functions. In comparison to other available sequencing methods, nanopore sequencing stands out due to its unique advantages of processing long nucleic acid strands in real time, within a small portable device, enabling the rapid analysis of samples in diverse settings. Evolving over the past decade, nanopore sequencing remains in a state of ongoing development and refinement, resulting in persistent challenges in protocols and technology. This article employs an interdisciplinary approach, evaluating experimental and computational methods to address critical gaps in our understanding in order to maximize the information gain from this advancing technology. Here, we present both overview and analysis of all aspects of nanopore sequencing by providing statistically supported insights. Thus, we aim to provide fresh perspectives on nanopore sequencing and give comprehensive guidelines for the diverse challenges that frequently impede optimal experimental outcomes.

Indexed as

Nanopore SequencingSequence Analysis, DNADNAGenomicsHigh-Throughput Nucleotide SequencingHumansNanoporesSequence Analysis, RNADNA

Identifiers

PMID41626694
PMCPMC12862388

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.