Evidence map›Paper›PMID 41626318›Full record

ArticleFrontiers in plant science2025

Isolation and transformation of perennial ryegrass (

Ferenz Sustek-Sánchez, Erki Eelmets, Lenne Nigul, Kairi Kärblane, Martin Laasmaa, Madara Balode-Sausina, Sanda Astra Berzina, Davis Ducis, Elza Kaktina, Kristina Jaškūne and 3 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Frontiers in plant science, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Ferenz Sustek-SánchezDepartment of Chemistry and Biotechnology, Tallinn University of Technology, Tallinn, Estonia.
Erki EelmetsDepartment of Chemistry and Biotechnology, Tallinn University of Technology, Tallinn, Estonia.
Lenne NigulDepartment of Chemistry and Biotechnology, Tallinn University of Technology, Tallinn, Estonia.
Kairi KärblaneDepartment of Chemistry and Biotechnology, Tallinn University of Technology, Tallinn, Estonia.
Martin LaasmaaDepartment of Cybernetics, Tallinn University of Technology, Tallinn, Estonia.
Madara Balode-SausinaDepartment of Molecular Life Sciences, Microbiology and Biotechnology, Faculty of Medicine and Life Sciences, University of Latvia, Riga, Latvia.
Sanda Astra BerzinaDepartment of Molecular Life Sciences, Microbiology and Biotechnology, Faculty of Medicine and Life Sciences, University of Latvia, Riga, Latvia.
Davis DucisDepartment of Molecular Life Sciences, Microbiology and Biotechnology, Faculty of Medicine and Life Sciences, University of Latvia, Riga, Latvia.
Elza KaktinaDepartment of Molecular Life Sciences, Microbiology and Biotechnology, Faculty of Medicine and Life Sciences, University of Latvia, Riga, Latvia.
Kristina JaškūneLaboratory of Genetics and Physiology, Lithuanian Research Centre for Agriculture and Forestry, Akademija, Lithuania.
Odd Arne RognliDepartment of Plant Sciences, Faculty of Biosciences, Norwegian University of Life Sciences (NMBU), Ås, Norway.
Nils RostoksDepartment of Molecular Life Sciences, Microbiology and Biotechnology, Faculty of Medicine and Life Sciences, University of Latvia, Riga, Latvia.
Cecilia SarmientoDepartment of Chemistry and Biotechnology, Tallinn University of Technology, Tallinn, Estonia.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Protoplasts are broadly used to perform different cellular and genetic assays. Transformation of protoplasts requires isolation methods that generate a large number of intact cells suitable for downstream applications.

Indexed as

CRISPR-Casgene editingguide RNALolium perenne L.PEGperennial ryegrasspolyethylene glycolprotoplasts

Identifiers

PMID41626318
PMCPMC12856575

What OpenQuestion holds

Textmetadata
LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.