Evidence map›Paper›PMID 41624283›Full record

ArticleFrontiers in veterinary science2025

Establishment of an ELISA for detecting oocyst-derived

Xinran Peng, Tiantian Geng, Yu Wang, Fen Du, Junlong Zhao, Rui Fang, Bang Shen, Yanqin Zhou

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Article in Frontiers in veterinary science, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

8 authors.

Xinran Peng *National Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, China.
Tiantian Geng *National Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, China.
Yu WangNational Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, China.
Fen DuHubei Center for Animal Disease Prevention and Control, Wuhan, Hubei, China.
Junlong ZhaoNational Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, China.
Rui FangNational Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, China.
Bang ShenNational Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, China.
Yanqin ZhouNational Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Background: Ovine toxoplasmosis is a zoonotic disease that severely impacts the development of the sheep industry. The primary routes of Methods: In this study, we initially screened eight proteins that are highly specifically expressed during the oocyst stage. Through Western blot (WB) analysis, we identified a protein (TGME49_267410) that could serve as a diagnostic antigen. Subsequently, we optimized the conditions for an indirect enzyme-linked immunosorbent assay (iELISA) using TGME49_267410. Based on these optimized conditions, we collected 1,350 sheep serum samples from various prefecture-level cities in Hubei Province and compared the detection results using both GRA1-iELISA and Results: In this study, we successfully identified TGME49_267410 as a specific diagnostic antigen for Conclusion: To summarize, the

Indexed as

ELISAepidemiological investigationoocyst infectionovine toxoplasmosisTGME49_2674106Toxoplasma gondii

Identifiers

PMID41624283
PMCPMC12853370

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