Evidence map›Paper›PMID 41623621›Full record

ArticleFrontiers in microbiology2025

Phosphorylation-driven conformational switching of the ArnA-ArnB complex involved in archaeal motility regulation.

Mohamed Watad, Lukas Korf, Wieland Steinchen, Filipp Bezold, Marian S Vogt, Po Hsun Wang, Leon Selbach, Sebastian Hepp, Luis Gayermann, Marleen van Wolferen and 3 more

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In one paragraph

Article in Frontiers in microbiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Journal of bacteriology · 2026
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Mohamed Watad *Department of Chemistry, Philipps University of Marburg, Marburg, Germany.
Lukas Korf *Department of Chemistry, Philipps University of Marburg, Marburg, Germany.
Wieland SteinchenDepartment of Chemistry, Philipps University of Marburg, Marburg, Germany.
Filipp BezoldDepartment of Chemistry, Philipps University of Marburg, Marburg, Germany.
Marian S VogtDepartment of Chemistry, Philipps University of Marburg, Marburg, Germany.
Po Hsun WangDepartment of Chemistry, Philipps University of Marburg, Marburg, Germany.
Leon SelbachDepartment of Chemistry, Philipps University of Marburg, Marburg, Germany.
Sebastian HeppDepartment of Chemistry, Philipps University of Marburg, Marburg, Germany.
Luis GayermannInstitute for Biology, Molecular Biology of Archaea, University of Freiburg, Freiburg, Germany.
Marleen van WolferenInstitute for Biology, Molecular Biology of Archaea, University of Freiburg, Freiburg, Germany.
Xing YeInstitute for Biology, Molecular Biology of Archaea, University of Freiburg, Freiburg, Germany.
Sonja-Verena AlbersInstitute for Biology, Molecular Biology of Archaea, University of Freiburg, Freiburg, Germany.
Lars-Oliver EssenDepartment of Chemistry, Philipps University of Marburg, Marburg, Germany.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

ArnA and ArnB serve as regulators within the Sulfolobus archaellum regulatory network by modulating the archaellum components ArlB and ArlX, which are essential for swimming motility. Together, they form a dynamic complex that, depending on nutrient availability, exists in either a loose, unphosphorylated or a tight, phosphorylated state. This transition is directed by phosphorylation via the kinase ArnC. To investigate this transition, we determined the cocrystal structure of the ArnA/ArnB complex, revealing that the zinc finger domain of ArnA interacts with both the β-sandwich and the C-terminal domains of ArnB. HDX data support the phosphorylation-dependent transition from a loose to a tight ArnAB complex driven by sequential phosphorylation of ArnB. This modification exposes the interaction surface of the C-terminal domain of ArnB, which then binds to the forkhead-associated domain of ArnA. Upon starvation of deletion strains of

Indexed as

archaeaArnBmotilityorder to disorderregulationsequential phosphorylationstarvation

Identifiers

PMID41623621
PMCPMC12852372

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.